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Anti-Human Granzyme B Rabbit Recombinant Antibody, PBS Only

Granzyme B Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240761G4

Cat no : 98070-1-PBS

Print datasheet

Synonyms

GZMB, CSP B, CGL1, CGL 1, CCPI

验证数据展示

  • FC experiment of human PBMCs using 98070-1-RR (same clone as 98070-1-PBS)

    FC experiment of human PBMCs using 98070-1-RR (same clone as 98070-1-PBS)

    1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human Granzyme B Rabbit Recombinant Antibody (98070-1-RR, Clone:240761G4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), and 0.25 ug APC Anti-Human CD8a (OKT8) (APC-65135, Clone: OKT8), and 0.25 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98070-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98070-1-RR (same clone as 98070-1-PBS)

    FC experiment of human PBMCs using 98070-1-RR (same clone as 98070-1-PBS)

    1x10^6 human PBMCs were stained with APC Anti-Human CD8a (OKT8) (APC-65135, Clone: OKT8) and 0.25 ug Anti-Human Granzyme B Rabbit Recombinant Antibody (98070-1-RR, Clone:240761G4), or Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98070-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98070-1-RR

    Affinity and Kinetic Characterization of 98070-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98070-1-RR against Human Granzyme B were performed. The affinity constant is 0.144 nM.

产品信息

98070-1-PBS targets Granzyme B in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
Synonyms GZMB, CSP B, CGL1, CGL 1, CCPI
Calculated Molecular Weight 247 aa, 28 kDa
GenBank Accession NumberBC030195
Gene Symbol Granzyme B
Gene ID (NCBI) 3002
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP10144
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). This full length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and high mannose glycosylated is 32 kDa and only 32kDa or smaller forms of granzyme B are accumulated within nuclei (PMID:8626751). GzmB also forms dimers.