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验证数据展示

  • IF Staining of HepG2 using CL488-67867

    IF Staining of HepG2 using CL488-67867

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 HABP2 antibody (CL488-67867, Clone: 1A7C2 ) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HepG2 using CL488-67867

    FC experiment of HepG2 using CL488-67867

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated HABP2 Monoclonal antibody (CL488-67867, Clone:1A7C2)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2b Isotype Control (MPC-11) (CL488-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated HABP2 Monoclonal antibody

HABP2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67867

产品说明书

CloneNo. 1A7C2

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

FSAP, Factor VII-activating protease, Factor VII activating protease, Factor seven-activating protease, EC:3.4.21.-

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67867-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67867 targets HABP2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 HABP2 fusion protein Ag3981 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 hyaluronan binding protein 2
别名 FSAP, Factor VII-activating protease, Factor VII activating protease, Factor seven-activating protease, EC:3.4.21.-
计算分子量 560 aa, 63 kDa
观测分子量60,63 kDa
GenBank蛋白编号BC031412
基因名称 HABP2
Gene ID (NCBI) 3026
RRIDAB_3672965
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14520
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HABP2 is also named as HGFAL, PHBP and belongs to the peptidase S1 family. It can activate coagulation factor VII and prourokinase and release the vasoactive peptide bradykinin by cleaving kininogen and is an important role in the regulation of the haemostasis system as well as fibroproliferative inflammatory processes (PMID:21655671). HABP2 is present as the 70 kDa inactive single chain precursor in human plasma and transforms to the active two chain form, 50 kDa heavy chain and 27 kDa light chain,and the 50 kDa heavy chain changes to two 26 kDa fragments, and 27 kDa light chain to 17 kDa and 8 kDa fragments, which are all bridged by disulfide linkages, by autocleavage(PMID:11217080).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HABP2 antibody CL488-67867Download protocol
FC protocol for CL Plus 488 HABP2 antibody CL488-67867Download protocol
Standard Protocols
Click here to view our Standard Protocols