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验证数据展示

  • WB analysis using 84374-4-RR

    WB analysis using 84374-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84374-4-RR (HAPLN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84374-4-RR

    Affinity and Kinetic Characterization of 84374-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84374-4-RR against Human HAPLN1 were performed. The affinity constant is below 1 pM.

HAPLN1 Recombinant antibody

HAPLN1 Recombinant Antibody for WB, ELISA
Cat No. 84374-4-RR

产品说明书

CloneNo. 241588E9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Proteoglycan link protein, CRTL1, CRT1, Cartilage-linking protein 1, Cartilage-link protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84374-4-RR targets HAPLN1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HAPLN1 fusion protein Ag25221 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hyaluronan and proteoglycan link protein 1
别名 Proteoglycan link protein, CRTL1, CRT1, Cartilage-linking protein 1, Cartilage-link protein
计算分子量 40 kDa
观测分子量40-45 kDa
GenBank蛋白编号BC057808
基因名称 HAPLN1
Gene ID (NCBI) 1404
RRIDAB_3671910
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP10915
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HAPLN1 (hyaluronan and proteoglycan link protein 1), also known as CRTL1. It is expected to be located in the cytoplasm and extracellular, and the protein is mainly expressed in placenta and small intestine. The calculated molecular weight of HAPLN1 is 40 kDa. HAPLN1 binds to aggrecan on the hyaluronic chain, stabilizing the aggregates and thus increasing compression resistance and shock absorption. HAPLN1 also functions as a growth factor, up regulating aggrecan and type II collagen synthesis in cartilage. It seems to be an essential part of cartilage homeostasis and may also contribute to homeostasis in the disc tissue (PMID: 23537453). It is reported in the literature that it is a double band.(PMID: 26341258)

实验方案

Product Specific Protocols
WB protocol for HAPLN1 antibody 84374-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols