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验证数据展示

  • WB analysis using 84374-4-RR

    WB analysis using 84374-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84374-4-RR (HAPLN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84374-4-RR

    Affinity and Kinetic Characterization of 84374-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84374-4-RR against Human HAPLN1 were performed. The affinity constant is below 1 pM.

HAPLN1 Recombinant antibody

HAPLN1 Recombinant Antibody for WB, ELISA
Cat No. 84374-4-RR

Print datasheet

CloneNo. 241588E9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Proteoglycan link protein, CRTL1, CRT1, Cartilage-linking protein 1, Cartilage-link protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84374-4-RR targets HAPLN1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen HAPLN1 fusion protein Ag25221 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name hyaluronan and proteoglycan link protein 1
Synonyms Proteoglycan link protein, CRTL1, CRT1, Cartilage-linking protein 1, Cartilage-link protein
Calculated Molecular Weight 40 kDa
Observed Molecular Weight40-45 kDa
GenBank Accession NumberBC057808
Gene Symbol HAPLN1
Gene ID (NCBI) 1404
RRIDAB_3671910
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP10915
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HAPLN1 (hyaluronan and proteoglycan link protein 1), also known as CRTL1. It is expected to be located in the cytoplasm and extracellular, and the protein is mainly expressed in placenta and small intestine. The calculated molecular weight of HAPLN1 is 40 kDa. HAPLN1 binds to aggrecan on the hyaluronic chain, stabilizing the aggregates and thus increasing compression resistance and shock absorption. HAPLN1 also functions as a growth factor, up regulating aggrecan and type II collagen synthesis in cartilage. It seems to be an essential part of cartilage homeostasis and may also contribute to homeostasis in the disc tissue (PMID: 23537453). It is reported in the literature that it is a double band.(PMID: 26341258)

实验方案

Product Specific Protocols
WB protocol for HAPLN1 antibody 84374-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols