验证数据展示
经过测试的应用
| Positive IF/ICC detected in | MCF-7 cells |
For other applications, we recommend the unconjugated version of this antibody, 67662-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67662 targets HARS in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | HARS fusion protein Ag9224 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | histidyl-tRNA synthetase |
| 别名 | Histidyl-tRNA synthetase, Histidine--tRNA ligase, cytoplasmic, HisRS, HARS1, EC:6.1.1.21 |
| 计算分子量 | 509 aa, 57 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC011807 |
| 基因名称 | HARS |
| Gene ID (NCBI) | 3035 |
| RRID | AB_3672957 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P12081 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HARS is a cytoplasmic enzyme that belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. HARS is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 HARS antibody CL488-67662 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |