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HAT1 Monoclonal antibody

HAT1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A11F6

Cat no : 67971-1-Ig

Print datasheet

Synonyms

KAT1, EC:2.3.1.48, 1A11F6

验证数据展示

  • WB analysis using 67971-1-Ig

    WB analysis using 67971-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67971-1-Ig (HAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HEK-293 using 67971-1-Ig

    WB analysis of HEK-293 using 67971-1-Ig

    WB result of HAT1 antibody (67971-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HAT1 transfected HEK-293 cells.

  • IHC staining of rat testis using 67971-1-Ig

    IHC staining of rat testis using 67971-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67971-1-Ig (HAT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HAT1 antibody included

经过测试的应用

Positive WB detected inLNCaP cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inrat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HAT1 Ready-To-Use IHC Kit. HAT1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67971-1-Ig targets HAT1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen HAT1 fusion protein Ag1952 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name histone acetyltransferase 1
Synonyms KAT1, EC:2.3.1.48, 1A11F6
Calculated Molecular Weight 419 aa, 50 kDa
Observed Molecular Weight45-46 kDa
GenBank Accession NumberBC018682
Gene Symbol HAT1
Gene ID (NCBI) 8520
RRIDAB_2918721
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO14929
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HAT1 antibody 67971-1-IgDownload protocol
IHC protocol for HAT1 antibody 67971-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols