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验证数据展示

  • FC experiment of HepG2 using CL488-67971

    FC experiment of HepG2 using CL488-67971

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human HAT1 (CL488-67971, Clone:1A11F6) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated HAT1 Monoclonal antibody

HAT1 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67971

Print datasheet

CloneNo. 1A11F6

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Synonyms

KAT1, EC:2.3.1.48, 1A11F6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HAT1 Ready-To-Use IHC Kit. HAT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67971-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67971 targets HAT1 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, mouse, rat
Immunogen HAT1 fusion protein Ag1952 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name histone acetyltransferase 1
Synonyms KAT1, EC:2.3.1.48, 1A11F6
Calculated Molecular Weight 419 aa, 50 kDa
Observed Molecular Weight45-46 kDa
GenBank Accession NumberBC018682
Gene Symbol HAT1
Gene ID (NCBI) 8520
RRIDAB_2923843
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO14929
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 HAT1 antibody CL488-67971Download protocol
Standard Protocols
Click here to view our Standard Protocols