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HBA1 Recombinant antibody, PBS Only

HBA1 Uni-rAbTM Recombinant Antibody for ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

ELISA, WB

Conjugate

Unconjugated

CloneNo.

240049F12

Cat no : 83185-5-PBS

Print datasheet

Synonyms

Hemoglobin Alpha, Hemoglobin a, HBA2, Alpha-globin, Alpha globin

验证数据展示

  • WB analysis of human placenta using 83185-5-RR (same clone as 83185-5-PBS)

    WB analysis of human placenta using 83185-5-RR (same clone as 83185-5-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 83185-5-RR (HBA1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83185-5-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 83185-5-RR (same clone as 83185-5-PBS)

    FC experiment of K-562 using 83185-5-RR (same clone as 83185-5-PBS)

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug Hba1 Recombinant Antibody (83185-5-RR, Clone:240049F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83185-5-PBS in a different storage buffer formulation.

  • FC experiment of TF-1 using 83185-5-RR (same clone as 83185-5-PBS)

    FC experiment of TF-1 using 83185-5-RR (same clone as 83185-5-PBS)

    1x10^6 TF-1 cells were intracellularly stained with 0.25 ug Hba1 Recombinant Antibody (83185-5-RR, Clone:240049F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83185-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83185-5-RR

    Affinity and Kinetic Characterization of 83185-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83185-5-RR against Human HBA1 were performed. The affinity constant is 3.94 nM.

产品信息

The immunogen of 83185-5-PBS is HBA1 Fusion Protein expressed in E. coli.

Tested Applications ELISA, WB Application Description
Tested Reactivity human
Immunogen HBA1 fusion protein Ag6040 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name hemoglobin, alpha 1
Synonyms Hemoglobin Alpha, Hemoglobin a, HBA2, Alpha-globin, Alpha globin
Calculated Molecular Weight 15 kDa
GenBank Accession NumberBC050661
Gene Symbol HBA1
Gene ID (NCBI) 3039
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP69905
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C