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HBA1 Recombinant antibody

HBA1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240049F12

Cat no : 83185-5-RR

Print datasheet

Synonyms

Hemoglobin Alpha, Hemoglobin a, HBA2, Alpha-globin, Alpha globin

验证数据展示

  • WB analysis of human placenta using 83185-5-RR

    WB analysis of human placenta using 83185-5-RR

    human placenta tissue were subjected to SDS PAGE followed by western blot with 83185-5-RR (HBA1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • FC experiment of K-562 using 83185-5-RR

    FC experiment of K-562 using 83185-5-RR

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug Hba1 Recombinant Antibody (83185-5-RR, Clone:240049F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of TF-1 using 83185-5-RR

    FC experiment of TF-1 using 83185-5-RR

    1x10^6 TF-1 cells were intracellularly stained with 0.25 ug Hba1 Recombinant Antibody (83185-5-RR, Clone:240049F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83185-5-RR

    Affinity and Kinetic Characterization of 83185-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83185-5-RR against Human HBA1 were performed. The affinity constant is 3.94 nM.

经过测试的应用

Positive WB detected inhuman placenta tissue
Positive FC (Intra) detected inK-562 cells, TF-1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83185-5-RR is HBA1 Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen HBA1 fusion protein Ag6040 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name hemoglobin, alpha 1
Synonyms Hemoglobin Alpha, Hemoglobin a, HBA2, Alpha-globin, Alpha globin
Calculated Molecular Weight 15 kDa
Observed Molecular Weight14-16 kDa, 27 kDa
GenBank Accession NumberBC050661
Gene Symbol HBA1
Gene ID (NCBI) 3039
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP69905
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). The antibody is specific to HBA1and has no cross reaction to other globin member.

实验方案

Product Specific Protocols
WB protocol for HBA1 antibody 83185-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols