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验证数据展示

  • WB analysis using 68146-1-Ig

    WB analysis using 68146-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68146-1-Ig (HBS1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 68146-1-Ig

    WB analysis using 68146-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68146-1-Ig (HBS1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 68146-1-Ig

    WB analysis of HEK-293 using 68146-1-Ig

    WB result of HBS1L antibody (68146-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HBS1L transfected HEK-293 cells.

  • IF Staining of U2OS using 68146-1-Ig

    IF Staining of U2OS using 68146-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using HBS1L antibody (68146-1-Ig, Clone: 1A10C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of U2OS using 68146-1-Ig

    IF Staining of U2OS using 68146-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using HBS1L antibody (68146-1-Ig, Clone: 1A10C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO验证

HBS1L Monoclonal antibody

HBS1L Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68146-1-Ig

产品说明书

CloneNo. 1A10C4

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

DKFZp686L13262, EF 1a, ERFS, HBS1, HBS1 like (S. cerevisiae), HBS1 like protein, HBS1L, HSPC276, KIAA1038

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inNCI-H1299 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, LNCaP cells, HeLa cells
Positive IF/ICC detected inU2OS cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68146-1-Ig targets HBS1L in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 HBS1L fusion protein Ag0388 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 HBS1-like (S. cerevisiae)
别名 DKFZp686L13262, EF 1a, ERFS, HBS1, HBS1 like (S. cerevisiae), HBS1 like protein, HBS1L, HSPC276, KIAA1038
计算分子量 75 kDa
观测分子量75 kDa
GenBank蛋白编号BC001465
基因名称 HBS1L
Gene ID (NCBI) 10767
RRIDAB_2923670
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y450
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HBS1L (HBS1-like), also known as EF-1a or ERFS, is a 684 amino acid protein that belongs to the GTP-binding elongation factor family and exists as multiple alternatively spliced isoforms. Expressed in kidney, brain, heart, placenta, liver, muscle and pancreas, HSB1L is thought to play a role in controlling fetal hemoglobin levels, specifically influencing platelet, monocyte and erythrocyte hemoglobin content. The gene encoding HBS1L maps to a locus on human chromosome 6 that is associated with sickle cell anemia and beta-thalassemia, suggesting a role for HBS1L in the pathogenesis of blood disorders.This antibody specifically recognizes the 75kd human HBS1L protein.

实验方案

Product Specific Protocols
WB protocol for HBS1L antibody 68146-1-IgDownload protocol
IF protocol for HBS1L antibody 68146-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols