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HBS1L Monoclonal antibody, PBS Only

HBS1L Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A10C4

Cat no : 68146-1-PBS

Print datasheet

Synonyms

DKFZp686L13262, EF 1a, ERFS, HBS1, HBS1 like (S. cerevisiae), HBS1 like protein, HBS1L, HSPC276, KIAA1038

验证数据展示

  • WB analysis using 68146-1-Ig (same clone as 68146-1-PBS)

    WB analysis using 68146-1-Ig (same clone as 68146-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68146-1-Ig (HBS1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68146-1-PBS in a different storage buffer formulation.

  • WB analysis using 68146-1-Ig (same clone as 68146-1-PBS)

    WB analysis using 68146-1-Ig (same clone as 68146-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68146-1-Ig (HBS1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68146-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 68146-1-Ig (same clone as 68146-1-PBS)

    WB analysis of HEK-293 using 68146-1-Ig (same clone as 68146-1-PBS)

    WB result of HBS1L antibody (68146-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HBS1L transfected HEK-293 cells. This data was developed using the same antibody clone with 68146-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 68146-1-Ig (same clone as 68146-1-PBS)

    IF Staining of U2OS using 68146-1-Ig (same clone as 68146-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using HBS1L antibody (68146-1-Ig, Clone: 1A10C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68146-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 68146-1-Ig (same clone as 68146-1-PBS)

    IF Staining of U2OS using 68146-1-Ig (same clone as 68146-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using HBS1L antibody (68146-1-Ig, Clone: 1A10C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68146-1-PBS in a different storage buffer formulation.

产品信息

68146-1-PBS targets HBS1L in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen HBS1L fusion protein Ag0388 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name HBS1-like (S. cerevisiae)
Synonyms DKFZp686L13262, EF 1a, ERFS, HBS1, HBS1 like (S. cerevisiae), HBS1 like protein, HBS1L, HSPC276, KIAA1038
Calculated Molecular Weight 75 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC001465
Gene Symbol HBS1L
Gene ID (NCBI) 10767
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y450
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HBS1L (HBS1-like), also known as EF-1a or ERFS, is a 684 amino acid protein that belongs to the GTP-binding elongation factor family and exists as multiple alternatively spliced isoforms. Expressed in kidney, brain, heart, placenta, liver, muscle and pancreas, HSB1L is thought to play a role in controlling fetal hemoglobin levels, specifically influencing platelet, monocyte and erythrocyte hemoglobin content. The gene encoding HBS1L maps to a locus on human chromosome 6 that is associated with sickle cell anemia and beta-thalassemia, suggesting a role for HBS1L in the pathogenesis of blood disorders.This antibody specifically recognizes the 75kd human HBS1L protein.