• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

HDAC1 antibody (mAb) (Clone 10E2)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB

CloneNo.

10E2

Cat No : 39881,39882 39881

Synonyms

HDAC1, AM-0131, 10E2, mAb, monoclonal, Histone Deacetylase 1, antibody, antibodies, hdac, deacetylase, sample

验证数据展示

  • HDAC1 antibody (mAb) tested by Western blot. Nuclear extract of HeLa cells (20 ug per lane) probed with HDAC1 antibody at a dilution of 1 ug/ml.

    HDAC1 antibody (mAb) tested by Western blot. Nuclear extract of HeLa cells (20 ug per lane) probed with HDAC1 antibody at a dilution of 1 ug/ml.

产品信息

Tested Applications WB

Applications Validated by Active Motif: WB*: 0.5 - 2 ug/ml dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. For ChIP-Seq analysis of HDAC1, we offer AbFlex HDAC1 Recombinant Antibody (rAb). For details, see Catalog No. 91215.
Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen This HDAC1 antibody was raised against a peptide corresponding to amino acids 467-482 of murine HDAC1.
Full Name HDAC1 antibody (mAb) (Clone 10E2)
Synonyms HDAC1, AM-0131, 10E2, mAb, monoclonal, Histone Deacetylase 1, antibody, antibodies, hdac, deacetylase, sample
Molecular weight 60 kDa
GenBank accession numberNP_004955
RRIDAB_2793378
Purification Method Protein G Chromatography
Buffer Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, a concentrated supernatant version (Catalog No. 39531) of this antibody is also available.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

HDAC1 (Histone Deacetylase 1, also designated HD1) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and HDAC10), class III (SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6 and SIRT-7) and class IV (HDAC11). HDAC1 and HDAC2 are recruited to Mad-Max complexes, which associate with the mSin3 scaffold protein, and are required for the transcriptional repression of Mad-Max target genes. HDAC1 is also involved in the regulation of p53. HDAC1 is expressed in various tissues. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both Histone H3 and Histone H4 in free histones or nucleosome substrate.