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CoraLite® Plus 647-conjugated HDAC2 Monoclonal antibody
HDAC2 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse
Applications
IF/ICC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
1A3E4
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy HDAC2 Ready-To-Use IHC Kit. HDAC2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67165-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-67165 targets HDAC2 in IF/ICC applications and shows reactivity with Human, mouse samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | Human, mouse |
Immunogen | HDAC2 fusion protein Ag21288 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | histone deacetylase 2 |
Synonyms | HD2, HDAC, HDAC2, histone deacetylase 2, RPD3, YAF1 |
Calculated Molecular Weight | 458 aa, 52 kDa; 488 aa,55 kDa |
Observed Molecular Weight | 55 kDa |
GenBank Accession Number | BC031055 |
Gene Symbol | HDAC2 |
Gene ID (NCBI) | 3066 |
RRID | AB_2920291 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q92769 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is raised against residues near the C terminus of human HDAC2.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 647 HDAC2 antibody CL647-67165 | Download protocol |
FC protocol for CL Plus 647 HDAC2 antibody CL647-67165 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |