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CoraLite® Plus 647-conjugated HDAC2 Monoclonal antibody

HDAC2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1A3E4

Cat no : CL647-67165

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Synonyms

HD2, HDAC, HDAC2, histone deacetylase 2, RPD3, YAF1



经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HDAC2 Ready-To-Use IHC Kit. HDAC2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67165-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-67165 targets HDAC2 in IF/ICC applications and shows reactivity with Human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, mouse
Immunogen HDAC2 fusion protein Ag21288 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name histone deacetylase 2
Synonyms HD2, HDAC, HDAC2, histone deacetylase 2, RPD3, YAF1
Calculated Molecular Weight458 aa, 52 kDa; 488 aa,55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC031055
Gene Symbol HDAC2
Gene ID (NCBI) 3066
RRIDAB_2920291
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92769
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is raised against residues near the C terminus of human HDAC2.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 HDAC2 antibody CL647-67165Download protocol
FC protocol for CL Plus 647 HDAC2 antibody CL647-67165Download protocol
Standard Protocols
Click here to view our Standard Protocols