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- KD/KO Validated
HDAC4 Monoclonal antibody
HDAC4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, IF-P, ELISA and More (3)
Conjugate
Unconjugated
CloneNo.
1D2G12
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse liver tissue |
Positive IF/ICC detected in | C2C12 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66838-1-Ig targets HDAC4 in WB, IHC, IF/ICC, IF-P, IP, ChIP, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
Cited Applications | WB, IF, IP, ChIP |
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Immunogen | HDAC4 fusion protein Ag11345 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | histone deacetylase 4 |
Synonyms | KIAA0288, HD4, EC:3.5.1.98, 1D2G12 |
Calculated Molecular Weight | 972 aa, 106 kDa |
Observed Molecular Weight | 140 kDa |
GenBank Accession Number | BC039904 |
Gene Symbol | HDAC4 |
Gene ID (NCBI) | 9759 |
RRID | AB_2882181 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P56524 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for HDAC4 antibody 66838-1-Ig | Download protocol |
IHC protocol for HDAC4 antibody 66838-1-Ig | Download protocol |
IF protocol for HDAC4 antibody 66838-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Oncogenesis USP38 regulates the stemness and chemoresistance of human colorectal cancer via regulation of HDAC3. | ||
Virulence Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions. | ||
J Mol Cell Cardiol Cellular heterogeneity and immune microenvironment revealed by single-cell transcriptome in venous malformation and cavernous venous malformation. | ||
Appl Physiol Nutr Metab Exercise improves high-fat diet-induced metabolic disorder by promoting HDAC5 degradation through the ubiquitin-proteasome system in skeletal muscle
| ||
J Toxicol Sci Propofol improves ischemia reperfusion-induced liver fibrosis by regulating lncRNA HOXA11-AS
| ||
Adv Clin Exp Med Tasquinimod enhances the sensitivity of ovarian cancer cells to cisplatin by regulating the Nur77-Bcl-2 apoptotic pathway |