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  • KD/KO Validated

HDAC4 Monoclonal antibody

HDAC4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA and More (3)

Conjugate

Unconjugated

CloneNo.

1D2G12

Cat no : 66838-1-Ig

Print datasheet

Synonyms

KIAA0288, HD4, EC:3.5.1.98, 1D2G12



经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue
Positive IF/ICC detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy HDAC4 Ready-To-Use IHC Kit. HDAC4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66838-1-Ig targets HDAC4 in WB, IHC, IF/ICC, IF-P, IP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Cited ApplicationsWB, IF, IP, ChIP
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen HDAC4 fusion protein Ag11345 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name histone deacetylase 4
Synonyms KIAA0288, HD4, EC:3.5.1.98, 1D2G12
Calculated Molecular Weight 972 aa, 106 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC039904
Gene Symbol HDAC4
Gene ID (NCBI) 9759
RRIDAB_2882181
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP56524
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HDAC4, also named as HDACA, belongs to the histone deacetylase family. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.

实验方案

Product Specific Protocols
WB protocol for HDAC4 antibody 66838-1-IgDownload protocol
IHC protocol for HDAC4 antibody 66838-1-IgDownload protocol
IF protocol for HDAC4 antibody 66838-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncogenesis

USP38 regulates the stemness and chemoresistance of human colorectal cancer via regulation of HDAC3.

Authors - Wei Zhan
humanIF

Virulence

Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions.

Authors - Wei Jie
humanIF

J Mol Cell Cardiol

Cellular heterogeneity and immune microenvironment revealed by single-cell transcriptome in venous malformation and cavernous venous malformation.

Authors - Yongyun Li
mouseWB,IP,ChIP

Appl Physiol Nutr Metab

Exercise improves high-fat diet-induced metabolic disorder by promoting HDAC5 degradation through the ubiquitin-proteasome system in skeletal muscle

Authors - Song Huang
  • KD Validated
mouseWB

J Toxicol Sci

Propofol improves ischemia reperfusion-induced liver fibrosis by regulating lncRNA HOXA11-AS

Authors - Jia Luo
  • KD Validated
human,mouseWB

Adv Clin Exp Med

Tasquinimod enhances the sensitivity of ovarian cancer cells to cisplatin by regulating the Nur77-Bcl-2 apoptotic pathway

Authors - Ying Lin