CoraLite® Plus 488-conjugated HDAC5 Polyclonal antibody
HDAC5 Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy HDAC5 Ready-To-Use IHC Kit. HDAC5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 29342-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-29342 targets HDAC5 in IF/ICC applications and shows reactivity with Human, mouse samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human, mouse |
| Immunogen | HDAC5 fusion protein Ag29122 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | histone deacetylase 5 |
| Synonyms | Antigen NY CO 9, FLJ90614, HD5, HDAC, HDAC5, histone deacetylase 5, KIAA0600, NY CO 9 |
| Calculated Molecular Weight | 122 kDa |
| Observed Molecular Weight | 120-140 kDa |
| GenBank Accession Number | NM_005474 |
| Gene Symbol | HDAC5 |
| Gene ID (NCBI) | 10014 |
| RRID | AB_3672838 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UQL6 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Histone acetylation and deacetylation alternately exposes and occludes DNA to transcription factors. At least 4 classes of HDAC were identified. HDAC5 is a class II HDAC. HDAC5 responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC5 is involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, HDAC5 shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 HDAC5 antibody CL488-29342 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |