HDAC6 Monoclonal antibody
HDAC6 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (1)
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1C7C3
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, NCI-H1299 cells, MCF-7 cells, HepG2 cells, Jurkat cells, K-562 cells, HL-60 cells, THP-1 cells |
Positive IHC detected in | human lung cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, SH-SY5Y cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IF | See 1 publications below |
产品信息
67250-1-Ig targets HDAC6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | Human |
Cited Reactivity | mouse |
Immunogen | HDAC6 fusion protein Ag28585 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | histone deacetylase 6 |
Synonyms | FLJ16239, HD6, HDAC, HDAC6, histone deacetylase 6, JM21, KIAA0901 |
Calculated Molecular Weight | 1063 aa, 114 kDa, 131 kDa |
Observed Molecular Weight | 150-160 kDa |
GenBank Accession Number | BC013737 |
Gene Symbol | HDAC6 |
Gene ID (NCBI) | 10013 |
RRID | AB_2882527 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9UBN7 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for HDAC6 antibody 67250-1-Ig | Download protocol |
IHC protocol for HDAC6 antibody 67250-1-Ig | Download protocol |
IF protocol for HDAC6 antibody 67250-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Tissue Res Upregulation of Profilin 2 on HDAC6 overexpression in mouse GC-1 cells and its putative role in germ cell migration in the testis |