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HDAC6 Monoclonal antibody, PBS Only

HDAC6 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C7C3

Cat no : 67250-1-PBS

Print datasheet

Synonyms

FLJ16239, HD6, HDAC, HDAC6, histone deacetylase 6, JM21, KIAA0901

验证数据展示

  • WB analysis using 67250-1-Ig (same clone as 67250-1-PBS)

    WB analysis using 67250-1-Ig (same clone as 67250-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67250-1-Ig (HDAC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67250-1-Ig (same clone as 67250-1-PBS)

    WB analysis of HeLa using 67250-1-Ig (same clone as 67250-1-PBS)

    WB result of HDAC6 antibody (67250-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HDAC6 transfected HeLa cells. This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67250-1-Ig (same clone as 67250-1-PBS)

    IHC staining of human breast cancer using 67250-1-Ig (same clone as 67250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67250-1-Ig (HDAC6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDAC6 antibody included
  • IHC staining of human lung cancer using 67250-1-Ig (same clone as 67250-1-PBS)

    IHC staining of human lung cancer using 67250-1-Ig (same clone as 67250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67250-1-Ig (HDAC6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDAC6 antibody included
  • IHC staining of human breast cancer using 67250-1-Ig (same clone as 67250-1-PBS)

    IHC staining of human breast cancer using 67250-1-Ig (same clone as 67250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67250-1-Ig (HDAC6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDAC6 antibody included
  • IHC staining of human breast cancer using 67250-1-Ig (same clone as 67250-1-PBS)

    IHC staining of human breast cancer using 67250-1-Ig (same clone as 67250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67250-1-Ig (HDAC6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDAC6 antibody included
  • IF Staining of SH-SY5Y using 67250-1-Ig (same clone as 67250-1-PBS)

    IF Staining of SH-SY5Y using 67250-1-Ig (same clone as 67250-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using HDAC6 antibody (67250-1-Ig, Clone: 1C7C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67250-1-Ig (same clone as 67250-1-PBS)

    IF Staining of HepG2 using 67250-1-Ig (same clone as 67250-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HDAC6 antibody (67250-1-Ig, Clone: 1C7C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy HDAC6 Ready-To-Use IHC Kit. HDAC6 primary antibody included.

产品信息

67250-1-PBS targets HDAC6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen HDAC6 fusion protein Ag28585 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name histone deacetylase 6
Synonyms FLJ16239, HD6, HDAC, HDAC6, histone deacetylase 6, JM21, KIAA0901
Calculated Molecular Weight 1063 aa, 114 kDa, 131 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberBC013737
Gene Symbol HDAC6
Gene ID (NCBI) 10013
RRIDAB_2882527
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UBN7
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC6 is a member of the class II mammalian histone deacetylases. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation. HDAC6 has some splicing variants such as P114(~130kd), P131(~160kd).This antibody is raised against residues near the C terminal of human HDAC6. The calculated molecular weight of HDAC6 is 130 kDa, but the modified the HDAC6 is about 150-160 kDa.