• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67250-1-Ig (same clone as 67250-1-PBS)

    WB analysis using 67250-1-Ig (same clone as 67250-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67250-1-Ig (HDAC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67250-1-Ig (same clone as 67250-1-PBS)

    WB analysis of HeLa using 67250-1-Ig (same clone as 67250-1-PBS)

    WB result of HDAC6 antibody (67250-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HDAC6 transfected HeLa cells. This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67250-1-Ig (same clone as 67250-1-PBS)

    IHC staining of human breast cancer using 67250-1-Ig (same clone as 67250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67250-1-Ig (HDAC6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDAC6 antibody included
  • IHC staining of human lung cancer using 67250-1-Ig (same clone as 67250-1-PBS)

    IHC staining of human lung cancer using 67250-1-Ig (same clone as 67250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67250-1-Ig (HDAC6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDAC6 antibody included
  • IHC staining of human breast cancer using 67250-1-Ig (same clone as 67250-1-PBS)

    IHC staining of human breast cancer using 67250-1-Ig (same clone as 67250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67250-1-Ig (HDAC6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDAC6 antibody included
  • IHC staining of human breast cancer using 67250-1-Ig (same clone as 67250-1-PBS)

    IHC staining of human breast cancer using 67250-1-Ig (same clone as 67250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67250-1-Ig (HDAC6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDAC6 antibody included
  • IF Staining of SH-SY5Y using 67250-1-Ig (same clone as 67250-1-PBS)

    IF Staining of SH-SY5Y using 67250-1-Ig (same clone as 67250-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using HDAC6 antibody (67250-1-Ig, Clone: 1C7C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67250-1-Ig (same clone as 67250-1-PBS)

    IF Staining of HepG2 using 67250-1-Ig (same clone as 67250-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HDAC6 antibody (67250-1-Ig, Clone: 1C7C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

HDAC6 Monoclonal antibody, PBS Only

HDAC6 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67250-1-PBS

产品说明书

CloneNo. 1C7C3

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, IF/ICC, ELISA

别名

FLJ16239, HD6, HDAC, HDAC6, histone deacetylase 6, JM21, KIAA0901

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy HDAC6 Ready-To-Use IHC Kit. HDAC6 primary antibody included.

产品信息

67250-1-PBS targets HDAC6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 HDAC6 fusion protein Ag28585 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 histone deacetylase 6
别名 FLJ16239, HD6, HDAC, HDAC6, histone deacetylase 6, JM21, KIAA0901
计算分子量 1063 aa, 114 kDa, 131 kDa
观测分子量 150-160 kDa
GenBank蛋白编号BC013737
基因名称 HDAC6
Gene ID (NCBI) 10013
RRIDAB_2882527
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UBN7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC6 is a member of the class II mammalian histone deacetylases. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation. HDAC6 has some splicing variants such as P114(~130kd), P131(~160kd).This antibody is raised against residues near the C terminal of human HDAC6. The calculated molecular weight of HDAC6 is 130 kDa, but the modified the HDAC6 is about 150-160 kDa.