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验证数据展示

  • IP experiment of Raji using 17548-1-AP (same clone as 17548-1-PBS)

    IP experiment of Raji using 17548-1-AP (same clone as 17548-1-PBS)

    IP result of anti-HDAC8 (IP:17548-1-AP, 4ug; Detection:17548-1-AP 1:600) with Raji cells lysate 2800ug. This data was developed using the same antibody clone with 17548-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 17548-1-AP (same clone as 17548-1-PBS)

    IF Staining of HeLa using 17548-1-AP (same clone as 17548-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17548-1-AP (HDAC8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17548-1-PBS in a different storage buffer formulation.

  • WB analysis using 17548-1-AP (same clone as 17548-1-PBS)

    WB analysis using 17548-1-AP (same clone as 17548-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17548-1-AP (HDAC8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17548-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 17548-1-AP (same clone as 17548-1-PBS)

    IHC staining of human lung cancer using 17548-1-AP (same clone as 17548-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17548-1-AP (HDAC8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17548-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDAC8 antibody included
  • IHC staining of human lung cancer using 17548-1-AP (same clone as 17548-1-PBS)

    IHC staining of human lung cancer using 17548-1-AP (same clone as 17548-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17548-1-AP (HDAC8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17548-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDAC8 antibody included
  • Featured Product
  • KD/KO验证

HDAC8 Polyclonal antibody, PBS Only

HDAC8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 17548-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

Protein decrotonylase HDAC8, Protein deacetylase HDAC8, HDAC 8, EC:3.5.1.98, EC:3.5.1.-

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HDAC8 Ready-To-Use IHC Kit. HDAC8 primary antibody included.

产品信息

17548-1-PBS targets HDAC8 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11696

Product name: Recombinant human HDAC8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-377 aa of BC050433

Sequence: MEEPEEPADSGQSLVPVYIYSPEYVSMCDSPAKIPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATFHTDAYLQHLQKVSQEGDDDHPDSIEYGLGYDCPATEGIFDYAAAIGGATITAAQCLIDGMCKVAINWSGGWHHAKKDEASGFCYLNDAVLGILRLRRKFERILYVDLDLHHGDGVEDAFSFTSKVMTVSLHKFSPGFFPGTGDVSDVGLGKGRYYSVNVPIQDGIQDEKYYQICESVLKEVYQAFNPKAVVLQLGADTIAGDPMCSFNMTPVGIGKCLKYILQWQLATLILGGGGYNLANTARCWTYLTGVILGKTLSSEIPDHEFFTAYGPDYVLEITPSCRPDRNEPHRIQQILNYIKGNLKHVV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histone deacetylase 8
别名 Protein decrotonylase HDAC8, Protein deacetylase HDAC8, HDAC 8, EC:3.5.1.98, EC:3.5.1.-
计算分子量 377 aa, 42 kDa
观测分子量 49 kDa
GenBank蛋白编号BC050433
基因名称 HDAC8
Gene ID (NCBI) 55869
RRIDAB_2116926
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BY41
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histone deacetylases (HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 8 was primarily found in the nucleus. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. This antibody is a rabbit polyclonal antibody raised against full length HDAC8 of human origin.

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