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HE4 Monoclonal antibody, PBS Only

HE4 Monoclonal Antibody for Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

5B2D9

Cat No : 66557-1-PBS

Print datasheet

Synonyms

HE4, WAP5, WFDC2

验证数据展示

  • IHC staining of human ovary tumor using 66557-1-Ig (same clone as 66557-1-PBS)

    IHC staining of human ovary tumor using 66557-1-Ig (same clone as 66557-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66557-1-Ig (HE4 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66557-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HE4 antibody included
  • IHC staining of human ovary tumor using 66557-1-Ig (same clone as 66557-1-PBS)

    IHC staining of human ovary tumor using 66557-1-Ig (same clone as 66557-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66557-1-Ig (HE4 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66557-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HE4 antibody included
  • IF Staining of SKOV-3 using 66557-1-Ig (same clone as 66557-1-PBS)

    IF Staining of SKOV-3 using 66557-1-Ig (same clone as 66557-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using HE4 antibody (66557-1-Ig, Clone: 5B2D9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66557-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy HE4 Ready-To-Use IHC Kit. HE4 primary antibody included.

产品信息

66557-1-PBS targets HE4 in IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human
Immunogen HE4 fusion protein Ag5936 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name WAP four-disulfide core domain 2
Synonyms HE4, WAP5, WFDC2
Calculated Molecular Weight 13 kDa
GenBank Accession NumberBC046106
Gene Symbol WFDC2
Gene ID (NCBI) 10406
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14508
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

WFDC2, also named as HE4, is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. HE4 is expressed in a number of normal tissues, and it is also highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. In some study, HE4 was referred to as a biomarker for ovarian carcinoma.