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HE4 Monoclonal antibody, PBS Only

HE4 Monoclonal Antibody for IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5B2D9

Cat no : 66557-1-PBS

Print datasheet

Synonyms

HE4, WAP5, WFDC2

验证数据展示

  • IHC staining of human ovary tumor using 66557-1-Ig (same clone as 66557-1-PBS)

    IHC staining of human ovary tumor using 66557-1-Ig (same clone as 66557-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66557-1-Ig (HE4 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66557-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HE4 antibody included
  • IHC staining of human ovary tumor using 66557-1-Ig (same clone as 66557-1-PBS)

    IHC staining of human ovary tumor using 66557-1-Ig (same clone as 66557-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66557-1-Ig (HE4 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66557-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HE4 antibody included
  • IF Staining of SKOV-3 using 66557-1-Ig (same clone as 66557-1-PBS)

    IF Staining of SKOV-3 using 66557-1-Ig (same clone as 66557-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using HE4 antibody (66557-1-Ig, Clone: 5B2D9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66557-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy HE4 Ready-To-Use IHC Kit. HE4 primary antibody included.

产品信息

66557-1-PBS targets HE4 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen HE4 fusion protein Ag5936 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name WAP four-disulfide core domain 2
Synonyms HE4, WAP5, WFDC2
Calculated Molecular Weight 13 kDa
GenBank Accession NumberBC046106
Gene Symbol HE4
Gene ID (NCBI) 10406
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14508
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WFDC2, also named as HE4, is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. HE4 is expressed in a number of normal tissues, and it is also highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. In some study, HE4 was referred to as a biomarker for ovarian carcinoma.