验证数据展示
经过测试的应用
Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | SKOV-3 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Immunohistochemistry (IHC) | IHC : 1:1000-1:5000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66557-1-Ig targets HE4 in IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
经测试应用 | IHC, IF/ICC, ELISA Application Description |
经测试反应性 | Human |
免疫原 | HE4 fusion protein Ag5936 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | WAP four-disulfide core domain 2 |
别名 | HE4, WAP5, WFDC2 |
计算分子量 | 13 kDa |
GenBank蛋白编号 | BC046106 |
基因名称 | HE4 |
Gene ID (NCBI) | 10406 |
RRID | AB_2881918 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q14508 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WFDC2, also named as HE4, is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. HE4 is expressed in a number of normal tissues, and it is also highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. In some study, HE4 was referred to as a biomarker for ovarian carcinoma.
实验方案
Product Specific Protocols | |
---|---|
IHC protocol for HE4 antibody 66557-1-Ig | Download protocol |
IF protocol for HE4 antibody 66557-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |