CoraLite® Plus 488-conjugated HE4 Monoclonal antibody
HE4 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
5B2D9
验证数据展示
经过测试的应用
Positive IF/ICC detected in | SKOV-3 cells |
Planning an IHC experiment? We recommend our IHCeasy HE4 Ready-To-Use IHC Kit. HE4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66557-1-Ig
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66557 targets HE4 in IF/ICC applications and shows reactivity with Human samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | Human |
Immunogen | HE4 fusion protein Ag5936 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | WAP four-disulfide core domain 2 |
Synonyms | dJ461P17.6, HE4, WAP5, WFDC2 |
Calculated Molecular Weight | 13 kDa |
GenBank Accession Number | BC046106 |
Gene Symbol | HE4 |
Gene ID (NCBI) | 10406 |
RRID | AB_2919343 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q14508 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WFDC2, also named as HE4, is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. HE4 is expressed in a number of normal tissues, and it is also highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. In some study, HE4 was referred to as a biomarker for ovarian carcinoma.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 HE4 antibody CL488-66557 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |