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HELLS Polyclonal antibody

HELLS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11955-1-AP

Print datasheet

Synonyms

Nbla10143, Lymphoid-specific helicase, Lymphoid specific helicase, LSH, helicase, lymphoid

验证数据展示

  • WB analysis of K-562 using 11955-1-AP

    WB analysis of K-562 using 11955-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 11955-1-AP

    IP experiment of mouse testis using 11955-1-AP

    IP result of anti-HELLS (IP:11955-1-AP, 4ug; Detection:11955-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

  • IHC staining of mouse thymus using 11955-1-AP

    IHC staining of mouse thymus using 11955-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse thymus using 11955-1-AP

    IHC staining of mouse thymus using 11955-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 11955-1-AP

    IHC staining of mouse testis using 11955-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 11955-1-AP

    IHC staining of mouse testis using 11955-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 11955-1-AP

    IHC staining of rat testis using 11955-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 11955-1-AP

    IHC staining of rat testis using 11955-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat thymus using 11955-1-AP

    IHC staining of rat thymus using 11955-1-AP

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat thymus using 11955-1-AP

    IHC staining of rat thymus using 11955-1-AP

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 11955-1-AP

    IF Staining of HepG2 using 11955-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HELLS antibody (11955-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 11955-1-AP

    FC experiment of HepG2 using 11955-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug HELLS Polyclonal antibody (11955-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inK-562 cells, DU 145 cells, mouse testis tissue, mouse thymus tissue, PC-3 cells
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse thymus tissue, mouse testis tissue, rat testis tissue, rat thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11955-1-AP targets HELLS in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen HELLS fusion protein Ag2558 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name helicase, lymphoid-specific
Synonyms Nbla10143, Lymphoid-specific helicase, Lymphoid specific helicase, LSH, helicase, lymphoid
Calculated Molecular Weight 348 aa, 40 kDa, 97 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC031004
Gene Symbol HELLS
Gene ID (NCBI) 3070
RRIDAB_2117529
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRZ9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lymphoid-specific helicase(HELLS) is a member of the SNF2 family of chromatin remodeling proteins that is required for development and survival. It's required for de novo or maintenance DNA methylation, which mediated the silencing of the imprinted CDKN1C gene. Also it involved in formation and organization of heterochromatin that regulate transcription and mitosis.

实验方案

Product Specific Protocols
WB protocol for HELLS antibody 11955-1-APDownload protocol
IHC protocol for HELLS antibody 11955-1-APDownload protocol
IF protocol for HELLS antibody 11955-1-APDownload protocol
IP protocol for HELLS antibody 11955-1-APDownload protocol
FC protocol for HELLS antibody 11955-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Nat Commun

Germinal center output is sustained by HELLS-dependent DNA-methylation-maintenance in B cells

Authors - Clara Cousu
View Article
mouseWB,IF

Nucleic Acids Res

The C-terminal 4CXXC-type zinc finger domain of CDCA7 recognizes hemimethylated DNA and modulates activities of chromatin remodeling enzyme HELLS

Authors - Akeo Shinkai
View Article
mouseWB

Sci Adv

CDCA7-associated global aberrant DNA hypomethylation translates to localized, tissue-specific transcriptional responses

Authors - Maja Vukic
View Article
mouseWB, IF

Genome Biol

Lsh regulates LTR retrotransposon repression independently of Dnmt3b function.

Authors - Donncha S Dunican
View Article
humanWB

mSystems

Adenovirus Remodeling of the Host Proteome and Host Factors Associated with Viral Genomes

Authors - Joseph M. Dybas
View Article

Hum Mol Genet

Converging disease genes in ICF syndrome: ZBTB24 controls expression of CDCA7 in mammals.

Authors - Haoyu Wu
View Article