验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-11955 targets HELLS in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Tested Reactivity | human |
Immunogen | HELLS fusion protein Ag2558 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | helicase, lymphoid-specific |
Synonyms | Nbla10143, Lymphoid-specific helicase, Lymphoid specific helicase, LSH, helicase, lymphoid |
Calculated Molecular Weight | 348 aa, 40 kDa, 97 kDa |
Observed Molecular Weight | 97 kDa |
GenBank Accession Number | BC031004 |
Gene Symbol | HELLS |
Gene ID (NCBI) | 3070 |
RRID | AB_3672521 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9NRZ9 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Lymphoid-specific helicase(HELLS) is a member of the SNF2 family of chromatin remodeling proteins that is required for development and survival. It's required for de novo or maintenance DNA methylation, which mediated the silencing of the imprinted CDKN1C gene. Also it involved in formation and organization of heterochromatin that regulate transcription and mitosis.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 HELLS antibody CL488-11955 | Download protocol |
FC protocol for CL Plus 488 HELLS antibody CL488-11955 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |