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验证数据展示

  • IF Staining of HepG2 using CL488-11955

    IF Staining of HepG2 using CL488-11955

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 HELLS antibody (CL488-11955) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-11955

    FC experiment of HepG2 using CL488-11955

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 HELLS Polyclonal Antibody (CL488-11955)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated HELLS Polyclonal antibody

HELLS Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-11955

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

Nbla10143, Lymphoid-specific helicase, Lymphoid specific helicase, LSH, helicase, lymphoid

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 11955-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11955 targets HELLS in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 HELLS fusion protein Ag2558 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 helicase, lymphoid-specific
别名 Nbla10143, Lymphoid-specific helicase, Lymphoid specific helicase, LSH, helicase, lymphoid
计算分子量 348 aa, 40 kDa, 97 kDa
观测分子量 97 kDa
GenBank蛋白编号BC031004
基因名称 HELLS
Gene ID (NCBI) 3070
RRIDAB_3672521
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRZ9
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lymphoid-specific helicase(HELLS) is a member of the SNF2 family of chromatin remodeling proteins that is required for development and survival. It's required for de novo or maintenance DNA methylation, which mediated the silencing of the imprinted CDKN1C gene. Also it involved in formation and organization of heterochromatin that regulate transcription and mitosis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HELLS antibody CL488-11955Download protocol
FC protocol for CL Plus 488 HELLS antibody CL488-11955Download protocol
Standard Protocols
Click here to view our Standard Protocols