Hephaestin Polyclonal antibody
Hephaestin Polyclonal Antibody for IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
11148-1-AP targets Hephaestin in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human, rat |
| Immunogen | Hephaestin fusion protein Ag1604 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | hephaestin |
| Synonyms | CPL, HEPH, Hephaestin, KIAA0698 |
| Calculated Molecular Weight | 130 kDa |
| GenBank Accession Number | BC011561 |
| Gene Symbol | Hephaestin |
| Gene ID (NCBI) | 9843 |
| RRID | AB_11064728 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BQS7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hephaestin, also named as KIAA0698, belongs to the multicopper oxidase family. It is provied as ferroxidase of cellular iron export. HEPH is implicated in iron homeostasis and may mediate iron efflux associated to ferroportin 1. HEPE is active in placenta but may not play a key role in placental iron transport.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Hephaestin antibody 11148-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Biochem Funct Ferroportin 1 and hephaestin expression in BeWo cell line with different iron treatment. | ||
Chin Herb Med Neuroprotective effects of kukoamine A on 6-OHDA-induced Parkinson's model through apoptosis and iron accumulation inhibition |