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验证数据展示

  • WB analysis using 60337-1-Ig

    WB analysis using 60337-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60337-1-Ig (HEXA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

HEXA Monoclonal antibody

HEXA Monoclonal Antibody for WB, ELISA
Cat No. 60337-1-Ig
CloneNo. 3A4F2

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, rabbit

Applications

WB, ELISA

Synonyms

HEXA, Hexosaminidase subunit A, TSD

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, rabbit testis tissue, A431 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60337-1-Ig targets HEXA in WB, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat, rabbit
Immunogen HEXA fusion protein Ag1854 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name hexosaminidase A (alpha polypeptide)
Synonyms HEXA, Hexosaminidase subunit A, TSD
Calculated Molecular Weight 529 aa, 60 kDa
Observed Molecular Weight 50-54 kDa, 60-67 kDa
GenBank Accession NumberBC018927
Gene Symbol HEXA
Gene ID (NCBI) 3073
RRIDAB_2881446
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06865
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta-hexosaminidase subunit alpha is involved in catalysis of degradation of the ganglioside GM2. Disfunction of beta-hexosaminidase leads to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. The enzyme is composed of two subunits, alpha and beta. This gene encodes alpha subunit of beta-hexosaminidase. Western blot with this antibody raised against HEXA detected 60 kDa (precursor) and 54 kDa (mature) size bands (PMID: 27682588, PMID: 30341570).

实验方案

Product Specific Protocols
WB protocol for HEXA antibody 60337-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols