HGS Monoclonal antibody

HGS Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67818-1-Ig

产品说明书

CloneNo. 3B10D6

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

HGS, HRS, Protein pp110, ZFYVE8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inLNCaP cells, PC-12 cells, Neuro-2a cells, Jurkat cells, A549 cells, HeLa cells, HEK-293 cells, K-562 cells, rat brain tissue, mouse brain tissue, HepG2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HGS Ready-To-Use IHC Kit. HGS primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67818-1-Ig targets HGS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 Human, Mouse, Rat
文献引用反应性human
免疫原 HGS fusion protein Ag28610 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 hepatocyte growth factor-regulated tyrosine kinase substrate
别名 HGS, HRS, Protein pp110, ZFYVE8
计算分子量 86 kDa
观测分子量110 kDa
GenBank蛋白编号BC003565
基因名称 HGS
Gene ID (NCBI) 9146
RRIDAB_2918581
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO14964
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS, synonyms: HRS, ZFYVE8) is a 110 to 115-kDa zinc finger phosphotyrosine protein inducible by stimulation with interleukin 2 (IL-2), granulocyte-macrophage colony-stimulating factor (GM-CSF) as well as hepatocyte growth factor (HGF), and is associated with signal-transducing adaptor molecule (STAM). HGS suppresses DNA synthesis upon stimulation with IL-2 and GM-CSF, counteracting the function of STAM which is critical for cell growth signaling mediated by the cytokines. HGS also interacts with the neurofibromatosis 2 tumor suppressor protein schwannomin/merlin. The growth suppression activity of schwannomin/merlin requires HGS and the binding of schwannomin/merlin to HGS facilitates its ability to function as a tumor suppressor, probably by inhibiting STAT activation.

实验方案

Product Specific Protocols
WB protocol for HGS antibody 67818-1-IgDownload protocol
IHC protocol for HGS antibody 67818-1-IgDownload protocol
IF protocol for HGS antibody 67818-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Mol Cancer

LAMTOR1 decreased exosomal PD-L1 to enhance immunotherapy efficacy in non-small cell lung cancer

Authors - Bo Wu
WB,IF

PLoS Pathog

Manganese is a potent inducer of lysosomal activity that inhibits de novo HBV infection

Authors - Lin Yu
Loading...