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HGS Monoclonal antibody, PBS Only

HGS Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3B10D6

Cat no : 67818-1-PBS

Print datasheet

Synonyms

HGS, HRS, Protein pp110, ZFYVE8

验证数据展示

  • WB analysis using 67818-1-Ig (same clone as 67818-1-PBS)

    WB analysis using 67818-1-Ig (same clone as 67818-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67818-1-Ig (HGS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67818-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67818-1-Ig (same clone as 67818-1-PBS)

    IHC staining of mouse brain using 67818-1-Ig (same clone as 67818-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67818-1-Ig (HGS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67818-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HGS antibody included
  • IHC staining of mouse brain using 67818-1-Ig (same clone as 67818-1-PBS)

    IHC staining of mouse brain using 67818-1-Ig (same clone as 67818-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67818-1-Ig (HGS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67818-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HGS antibody included
  • IF Staining of HepG2 using 67818-1-Ig (same clone as 67818-1-PBS)

    IF Staining of HepG2 using 67818-1-Ig (same clone as 67818-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HGS antibody (67818-1-Ig, Clone: 3B10D6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67818-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy HGS Ready-To-Use IHC Kit. HGS primary antibody included.

产品信息

67818-1-PBS targets HGS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen HGS fusion protein Ag28610 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name hepatocyte growth factor-regulated tyrosine kinase substrate
Synonyms HGS, HRS, Protein pp110, ZFYVE8
Calculated Molecular Weight 86 kDa
Observed Molecular Weight110 kDa
GenBank Accession NumberBC003565
Gene Symbol HGS
Gene ID (NCBI) 9146
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14964
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS, synonyms: HRS, ZFYVE8) is a 110 to 115-kDa zinc finger phosphotyrosine protein inducible by stimulation with interleukin 2 (IL-2), granulocyte-macrophage colony-stimulating factor (GM-CSF) as well as hepatocyte growth factor (HGF), and is associated with signal-transducing adaptor molecule (STAM). HGS suppresses DNA synthesis upon stimulation with IL-2 and GM-CSF, counteracting the function of STAM which is critical for cell growth signaling mediated by the cytokines. HGS also interacts with the neurofibromatosis 2 tumor suppressor protein schwannomin/merlin. The growth suppression activity of schwannomin/merlin requires HGS and the binding of schwannomin/merlin to HGS facilitates its ability to function as a tumor suppressor, probably by inhibiting STAT activation.