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HGS Monoclonal antibody, PBS Only

HGS Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3B10D6

Cat no : 67818-1-PBS

Print datasheet

Synonyms

HGS, HRS, Protein pp110, ZFYVE8

验证数据展示

  • WB analysis using 67818-1-Ig (same clone as 67818-1-PBS)

    WB analysis using 67818-1-Ig (same clone as 67818-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67818-1-Ig (HGS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67818-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67818-1-Ig (same clone as 67818-1-PBS)

    IHC staining of mouse brain using 67818-1-Ig (same clone as 67818-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67818-1-Ig (HGS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67818-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HGS antibody included
  • IHC staining of mouse brain using 67818-1-Ig (same clone as 67818-1-PBS)

    IHC staining of mouse brain using 67818-1-Ig (same clone as 67818-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67818-1-Ig (HGS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67818-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HGS antibody included
  • IF Staining of HepG2 using 67818-1-Ig (same clone as 67818-1-PBS)

    IF Staining of HepG2 using 67818-1-Ig (same clone as 67818-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HGS antibody (67818-1-Ig, Clone: 3B10D6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67818-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy HGS Ready-To-Use IHC Kit. HGS primary antibody included.

产品信息

67818-1-PBS targets HGS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen HGS fusion protein Ag28610 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name hepatocyte growth factor-regulated tyrosine kinase substrate
Synonyms HGS, HRS, Protein pp110, ZFYVE8
Calculated Molecular Weight 86 kDa
Observed Molecular Weight110 kDa
GenBank Accession NumberBC003565
Gene Symbol HGS
Gene ID (NCBI) 9146
RRIDAB_2918581
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14964
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS, synonyms: HRS, ZFYVE8) is a 110 to 115-kDa zinc finger phosphotyrosine protein inducible by stimulation with interleukin 2 (IL-2), granulocyte-macrophage colony-stimulating factor (GM-CSF) as well as hepatocyte growth factor (HGF), and is associated with signal-transducing adaptor molecule (STAM). HGS suppresses DNA synthesis upon stimulation with IL-2 and GM-CSF, counteracting the function of STAM which is critical for cell growth signaling mediated by the cytokines. HGS also interacts with the neurofibromatosis 2 tumor suppressor protein schwannomin/merlin. The growth suppression activity of schwannomin/merlin requires HGS and the binding of schwannomin/merlin to HGS facilitates its ability to function as a tumor suppressor, probably by inhibiting STAT activation.