• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

HIBCH Recombinant antibody, PBS Only (Detector)

HIBCH Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240590A3

Cat no : 83569-1-PBS

Print datasheet

Synonyms

HIB-CoA hydrolase, HIB CoA hydrolase, EC:3.1.2.4, 3-hydroxyisobutyryl-coenzyme A hydrolase, 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial

验证数据展示

  • WB analysis using 83569-1-RR (same clone as 83569-1-PBS)

    WB analysis using 83569-1-RR (same clone as 83569-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83569-1-RR (HIBCH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83569-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83569-1-RR (same clone as 83569-1-PBS)

    IF Staining of HepG2 using 83569-1-RR (same clone as 83569-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIBCH antibody (83569-1-RR, Clone: 240590A3 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83569-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83569-1-RR (same clone as 83569-1-PBS)

    FC experiment of HepG2 using 83569-1-RR (same clone as 83569-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug HIBCH Recombinant antibody (83569-1-RR, Clone:240590A3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83569-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00560-3

    Cytometric bead array standard curve of MP00560-3

    Cytometric bead array standard curve of MP00560-3, HIBCH Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83569-3-PBS. Detection antibody: 83569-1-PBS. Standard: Ag6149. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83569-1-RR

    Affinity and Kinetic Characterization of 83569-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83569-1-RR against Human HIBCH were performed. The affinity constant is 1.37 nM.

Planning an IHC experiment? We recommend our IHCeasy HIBCH Ready-To-Use IHC Kit. HIBCH primary antibody included.

产品信息

83569-1-PBS targets HIBCH as part of a matched antibody pair:

MP00560-3: 83569-3-PBS capture and 83569-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen HIBCH fusion protein Ag6149 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name 3-hydroxyisobutyryl-Coenzyme A hydrolase
Synonyms HIB-CoA hydrolase, HIB CoA hydrolase, EC:3.1.2.4, 3-hydroxyisobutyryl-coenzyme A hydrolase, 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial
Calculated Molecular Weight 43 kDa
GenBank Accession NumberBC067822
Gene Symbol HIBCH
Gene ID (NCBI) 26275
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6NVY1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C