验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A549 cells |
Planning an IHC experiment? We recommend our IHCeasy TGFB1I1/HIC5 Ready-To-Use IHC Kit. TGFB1I1/HIC5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10565-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-10565 targets HIC5 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | HIC5 fusion protein Ag0868 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | transforming growth factor beta 1 induced transcript 1 |
| Synonyms | ARA55, HIC 5, HIC5, TGFB1I1, TSC 5 |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 48-50 kDa |
| GenBank Accession Number | BC001830 |
| Gene Symbol | HIC5 |
| Gene ID (NCBI) | 7041 |
| RRID | AB_3672451 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43294 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 HIC5 antibody CL488-10565 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |