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HIF-1 alpha Recombinant antibody, PBS Only

HIF-1 alpha Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 80933-1-PBS

产品说明书

CloneNo. 2K1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

HIF1A, 2K1, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


产品信息

80933-1-PBS targets HIF-1 alpha in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 HIF-1 alpha fusion protein Ag15198 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
别名 HIF1A, 2K1, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1
计算分子量 826 aa, 93 kDa
观测分子量120 kDa
GenBank蛋白编号BC012527
基因名称 HIF1A
Gene ID (NCBI) 3091
RRIDAB_2918921
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16665
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, PMID: 7539918).

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