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  • Featured Product
  • KD/KO Validated

HIF-1 alpha Recombinant antibody

HIF-1 alpha Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2K1

Cat no : 80933-1-RR

Print datasheet

Synonyms

HIF1A, HIF 1 alpha, Class E basic helix-loop-helix protein 78, Basic-helix-loop-helix-PAS protein MOP1, ARNT-interacting protein

验证数据展示

  • WB analysis of HeLa using 80933-1-RR

    WB analysis of HeLa using 80933-1-RR

    Untreated and Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 80933-1-RR (HIF1a antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 80933-1-RR

    WB analysis of HeLa using 80933-1-RR

    WB result of HIF-1 alpha antibody (80933-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIF-1 alpha transfected HeLa cells. Sample 1: non-treated sh-Control transfected HeLa cells, Sample 2: Cobalt Chloride treated sh-Control transfected HeLa cells, Sample 3: Cobalt Chloride treated sh-HIF-1 alpha transfected HeLa cells.

  • IF Staining of HepG2 using 80933-1-RR

    IF Staining of HepG2 using 80933-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HepG2 cells using HIF1a antibody (80933-1-RR, Clone: 2K1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 80933-1-RR

    IF Staining of HeLa using 80933-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HeLa cells using HIF-1 alpha antibody (80933-1-RR, Clone: 2K1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IHC staining of human thyroid cancer using 80933-1-RR

    IHC staining of human thyroid cancer using 80933-1-RR

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 80933-1-RR (HIF1a antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human thyroid cancer using 80933-1-RR

    IHC staining of human thyroid cancer using 80933-1-RR

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 80933-1-RR (HIF1a antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 80933-1-RR

    Affinity and Kinetic Characterization of 80933-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80933-1-RR against Human HIF-1 alpha were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inCobalt Chloride treated HeLa cells, HeLa cells
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCobalt Chloride treated HeLa cells, Cobalt Chloride treated HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80933-1-RR targets HIF-1 alpha in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen HIF-1 alpha fusion protein Ag15198 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Synonyms HIF1A, HIF 1 alpha, Class E basic helix-loop-helix protein 78, Basic-helix-loop-helix-PAS protein MOP1, ARNT-interacting protein
Calculated Molecular Weight 826 aa, 93 kDa
Observed Molecular Weight120 kDa
GenBank Accession NumberBC012527
Gene Symbol HIF1A
Gene ID (NCBI) 3091
RRIDAB_2918921
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16665
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, PMID: 7539918).

实验方案

Product Specific Protocols
WB protocol for HIF-1 alpha antibody 80933-1-RRDownload protocol
IHC protocol for HIF-1 alpha antibody 80933-1-RRDownload protocol
IF protocol for HIF-1 alpha antibody 80933-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols