• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of U2OS using CL488-68231

    IF Staining of U2OS using CL488-68231

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 HIGD1A antibody (CL488-68231, Clone: 3A8E2 ) at dilution of 1:400.

  • FC experiment of U2OS using CL488-68231

    FC experiment of U2OS using CL488-68231

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated HIGD1A Monoclonal antibody (CL488-68231, Clone:3A8E2)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2b isotype control (11B8C4) (CL488-66360-3, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated HIGD1A Monoclonal antibody

HIGD1A Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68231

产品说明书

CloneNo. 3A8E2

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

RCF1 homolog A, Hypoxia-inducible gene 1 protein, HSPC010, HIG1 domain family member 1A, mitochondrial, HIG1 domain family member 1A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells
For other applications, we recommend the unconjugated version of this antibody, 68231-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68231 targets HIGD1A in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 HIGD1A fusion protein Ag14027 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 HIG1 hypoxia inducible domain family, member 1A
别名 RCF1 homolog A, Hypoxia-inducible gene 1 protein, HSPC010, HIG1 domain family member 1A, mitochondrial, HIG1 domain family member 1A
计算分子量 93 aa, 10 kDa
观测分子量10 kDa
GenBank蛋白编号BC000601
基因名称 HIGD1A
Gene ID (NCBI) 25994
RRIDAB_3672981
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y241
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIG1 domain family member 1A (HIGD1A) is a proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes (PMID:22342701). It is induced by hypoxia induction.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HIGD1A antibody CL488-68231Download protocol
FC protocol for CL Plus 488 HIGD1A antibody CL488-68231Download protocol
Standard Protocols
Click here to view our Standard Protocols