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HINT1 Monoclonal antibody, PBS Only

HINT1 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1G3E4

Cat No : 67583-1-PBS

Print datasheet

Synonyms

FLJ30414, FLJ32340, HINT, HINT1, PKCI 1, PKCI1, PRKCNH1, Protein kinase C inhibitor 1

验证数据展示

  • IHC staining of human breast cancer using 67583-1-Ig (same clone as 67583-1-PBS)

    IHC staining of human breast cancer using 67583-1-Ig (same clone as 67583-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67583-1-Ig (HINT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67583-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HINT1 antibody included
  • WB analysis using 67583-1-Ig (same clone as 67583-1-PBS)

    WB analysis using 67583-1-Ig (same clone as 67583-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67583-1-Ig (HINT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67583-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67583-1-Ig (same clone as 67583-1-PBS)

    IHC staining of human breast cancer using 67583-1-Ig (same clone as 67583-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67583-1-Ig (HINT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67583-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HINT1 antibody included
  • IF Staining of HeLa using 67583-1-Ig (same clone as 67583-1-PBS)

    IF Staining of HeLa using 67583-1-Ig (same clone as 67583-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HINT1 antibody (67583-1-Ig, Clone: 1G3E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67583-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy HINT1 Ready-To-Use IHC Kit. HINT1 primary antibody included.

产品信息

67583-1-PBS targets HINT1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human
Immunogen HINT1 fusion protein Ag16662 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name histidine triad nucleotide binding protein 1
Synonyms FLJ30414, FLJ32340, HINT, HINT1, PKCI 1, PKCI1, PRKCNH1, Protein kinase C inhibitor 1
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC007090
Gene Symbol HINT1
Gene ID (NCBI) 3094
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49773
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

HINT1(Histidine triad nucleotide-binding protein 1) is also named as HINT, PKCI1, PRKCNH1,which is a member of the histidine triad (HIT) family, highly conserved in diverse species and ubiquitously expressed in mammalian tissues.It hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.