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HINT1 Monoclonal antibody, PBS Only

HINT1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G3E4

Cat no : 67583-1-PBS

Print datasheet

Synonyms

FLJ30414, FLJ32340, HINT, HINT1, PKCI 1, PKCI1, PRKCNH1, Protein kinase C inhibitor 1

验证数据展示

  • IHC staining of human breast cancer using 67583-1-Ig (same clone as 67583-1-PBS)

    IHC staining of human breast cancer using 67583-1-Ig (same clone as 67583-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67583-1-Ig (HINT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67583-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HINT1 antibody included
  • WB analysis using 67583-1-Ig (same clone as 67583-1-PBS)

    WB analysis using 67583-1-Ig (same clone as 67583-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67583-1-Ig (HINT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67583-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67583-1-Ig (same clone as 67583-1-PBS)

    IHC staining of human breast cancer using 67583-1-Ig (same clone as 67583-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67583-1-Ig (HINT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67583-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HINT1 antibody included
  • IF Staining of HeLa using 67583-1-Ig (same clone as 67583-1-PBS)

    IF Staining of HeLa using 67583-1-Ig (same clone as 67583-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HINT1 antibody (67583-1-Ig, Clone: 1G3E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67583-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy HINT1 Ready-To-Use IHC Kit. HINT1 primary antibody included.

产品信息

67583-1-PBS targets HINT1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen HINT1 fusion protein Ag16662 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name histidine triad nucleotide binding protein 1
Synonyms FLJ30414, FLJ32340, HINT, HINT1, PKCI 1, PKCI1, PRKCNH1, Protein kinase C inhibitor 1
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC007090
Gene Symbol HINT1
Gene ID (NCBI) 3094
RRIDAB_2882793
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49773
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HINT1(Histidine triad nucleotide-binding protein 1) is also named as HINT, PKCI1, PRKCNH1,which is a member of the histidine triad (HIT) family, highly conserved in diverse species and ubiquitously expressed in mammalian tissues.It hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.