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  • Featured Product
  • KD/KO Validated

HIP1 Monoclonal antibody, PBS Only

HIP1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D3G3

Cat no : 67163-1-PBS

Print datasheet

Synonyms

HIP 1, HIP I, HIP1, ILWEQ

验证数据展示

  • WB analysis using 67163-1-Ig (same clone as 67163-1-PBS)

    WB analysis using 67163-1-Ig (same clone as 67163-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67163-1-Ig (HIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67163-1-Ig (same clone as 67163-1-PBS)

    WB analysis of HeLa using 67163-1-Ig (same clone as 67163-1-PBS)

    WB result of HIP1 antibody (67163-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIP1 transfected HeLa cells. This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67163-1-Ig (same clone as 67163-1-PBS)

    IHC staining of human colon cancer using 67163-1-Ig (same clone as 67163-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67163-1-Ig (HIP1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HIP1 antibody included
  • IHC staining of human colon cancer using 67163-1-Ig (same clone as 67163-1-PBS)

    IHC staining of human colon cancer using 67163-1-Ig (same clone as 67163-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67163-1-Ig (HIP1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HIP1 antibody included
  • IF Staining of human colon cancer using 67163-1-Ig (same clone as 67163-1-PBS)

    IF Staining of human colon cancer using 67163-1-Ig (same clone as 67163-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using HIP1 antibody (67163-1-Ig, Clone: 3D3G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy HIP1 Ready-To-Use IHC Kit. HIP1 primary antibody included.

产品信息

67163-1-PBS targets HIP1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse, pig samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity Human, mouse, pig
Immunogen HIP1 fusion protein Ag17982 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name huntingtin interacting protein 1
Synonyms HIP 1, HIP I, HIP1, ILWEQ
Calculated Molecular Weight 1037 aa, 116 kDa
Observed Molecular Weight 110-120 kDa
GenBank Accession NumberBC110545
Gene Symbol HIP1
Gene ID (NCBI) 3092
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00291
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HIP1 (huntingtin interacting protein-1) is a endocytic protein that plays a role in clathrin-mediated endocytosis and trafficking. HIP1 can interact with huntingtin (HTT) protein whose mutations cause Huntington's disease (HD), thus gets involved in neurodegeneration. By stabilizing receptor tyrosine kinases following ligand-induced endocytosis, HIP1 may act to promote cell survival. HIP1 is overexpressed in a variety of human cancers and may be a potential predictor for cancer progression and prognosis.