• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

HIP1 Monoclonal antibody

HIP1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3D3G3

Cat no : 67163-1-Ig

Print datasheet

Synonyms

HIP 1, HIP I, HIP1, ILWEQ

验证数据展示

  • WB analysis using 67163-1-Ig

    WB analysis using 67163-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67163-1-Ig (HIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67163-1-Ig

    WB analysis of HeLa using 67163-1-Ig

    WB result of HIP1 antibody (67163-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIP1 transfected HeLa cells.

  • IHC staining of human colon cancer using 67163-1-Ig

    IHC staining of human colon cancer using 67163-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67163-1-Ig (HIP1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HIP1 antibody included
  • IHC staining of human colon cancer using 67163-1-Ig

    IHC staining of human colon cancer using 67163-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67163-1-Ig (HIP1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HIP1 antibody included
  • IF Staining of human colon cancer using 67163-1-Ig

    IF Staining of human colon cancer using 67163-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using HIP1 antibody (67163-1-Ig, Clone: 3D3G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, pig brain tissue, mouse brain tissue, A549 cells, PC-3 cells
Positive IHC detected inhuman colon cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon cancer tissue
Planning an IHC experiment? We recommend our IHCeasy HIP1 Ready-To-Use IHC Kit. HIP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67163-1-Ig targets HIP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, pig samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity Human, mouse, pig
Immunogen HIP1 fusion protein Ag17982 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name huntingtin interacting protein 1
Synonyms HIP 1, HIP I, HIP1, ILWEQ
Calculated Molecular Weight 1037 aa, 116 kDa
Observed Molecular Weight 110-120 kDa
GenBank Accession NumberBC110545
Gene Symbol HIP1
Gene ID (NCBI) 3092
RRIDAB_2882459
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00291
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIP1 (huntingtin interacting protein-1) is a endocytic protein that plays a role in clathrin-mediated endocytosis and trafficking. HIP1 can interact with huntingtin (HTT) protein whose mutations cause Huntington's disease (HD), thus gets involved in neurodegeneration. By stabilizing receptor tyrosine kinases following ligand-induced endocytosis, HIP1 may act to promote cell survival. HIP1 is overexpressed in a variety of human cancers and may be a potential predictor for cancer progression and prognosis.

实验方案

Product Specific Protocols
WB protocol for HIP1 antibody 67163-1-IgDownload protocol
IHC protocol for HIP1 antibody 67163-1-IgDownload protocol
IF protocol for HIP1 antibody 67163-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols