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验证数据展示

  • IF Staining of A549 using CL594-67163

    IF Staining of A549 using CL594-67163

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite®594 HIP1 antibody (CL594-67163, Clone: 3D3G3 ) at dilution of 1:200, CL488-Phalloidin (green).

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  • KD/KO验证

CoraLite®594-conjugated HIP1 Monoclonal antibody

HIP1 Monoclonal Antibody for IF/ICC
Cat No. CL594-67163

产品说明书

CloneNo. 3D3G3

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, pig

应用

IF/ICC

别名

HIP 1, HIP I, HIP1, ILWEQ

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy HIP1 Ready-To-Use IHC Kit. HIP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67163-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67163 targets HIP1 in IF/ICC applications and shows reactivity with Human, mouse, pig samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, mouse, pig
免疫原 HIP1 fusion protein Ag17982 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 huntingtin interacting protein 1
别名 HIP 1, HIP I, HIP1, ILWEQ
计算分子量 1037 aa, 116 kDa
观测分子量 110-120 kDa
GenBank蛋白编号BC110545
基因名称 HIP1
Gene ID (NCBI) 3092
RRIDAB_2920092
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO00291
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIP1 (huntingtin interacting protein-1) is a endocytic protein that plays a role in clathrin-mediated endocytosis and trafficking. HIP1 can interact with huntingtin (HTT) protein whose mutations cause Huntington's disease (HD), thus gets involved in neurodegeneration. By stabilizing receptor tyrosine kinases following ligand-induced endocytosis, HIP1 may act to promote cell survival. HIP1 is overexpressed in a variety of human cancers and may be a potential predictor for cancer progression and prognosis.

实验方案

Product Specific Protocols
IF protocol for CL594 HIP1 antibody CL594-67163Download protocol
Standard Protocols
Click here to view our Standard Protocols