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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83194-4-RR (same clone as 83194-4-PBS)

    WB analysis using 83194-4-RR (same clone as 83194-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83194-4-RR (HIRA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83194-4-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 83194-4-RR (same clone as 83194-4-PBS)

    FC experiment of K-562 using 83194-4-RR (same clone as 83194-4-PBS)

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug HIRA Recombinant antibody (83194-4-RR, Clone:240006F8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83194-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83194-4-RR

    Affinity and Kinetic Characterization of 83194-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83194-4-RR against Human HIRA were performed. The affinity constant is below 1 pM.

HIRA Recombinant antibody, PBS Only

HIRA Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 83194-4-PBS

产品说明书

CloneNo. 240006F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

240006F8, HIR, HIRA/HIR, Protein HIRA

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83194-4-PBS targets HIRA in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原 HIRA fusion protein Ag34439 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 HIR histone cell cycle regulation defective homolog A (S. cerevisiae)
别名 240006F8, HIR, HIRA/HIR, Protein HIRA
计算分子量 112 kDa
观测分子量112 kDa
GenBank蛋白编号NM_003325
基因名称 HIRA
Gene ID (NCBI) 7290
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP54198
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HIRA protein is encoded by a gene within a region of human chromosome 22q11.2 deleted in most patients with the DiGeorge syndrome, a developmental disorder, and was named for its amino acid sequence homology to two S. cerevisiae proteins, Hir1p and Hir2p. HIRA is critical in a specific chromatin assembly pathway that takes place independently of DNA synthesis (PMID: 12049744). HIRA complex associates with various proteins, such as transcription factors, RNA pol II, Prohibitin (PHB), and replication protein A (RPA) suggests that the targeting of the complex at specific locations may exploit additional structural properties and mechanisms (PMID: 30082790).