验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, HEK-293 cells, HeLa cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, chicken brain tissue, zebrafish, wheat whole plant |
Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68503-1-Ig targets Histone H3 (C-terminal) in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, chicken, zebrafish, wheat samples.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | human, mouse, rat, chicken, zebrafish, wheat |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | histone cluster 1, H3a |
Synonyms | HIST1H3A, 2G10C3, Histone-H3, HistoneH3, Histone H3 |
Observed Molecular Weight | 15 kDa |
GenBank Accession Number | BC066245 |
Gene Symbol | HIST1H3A |
Gene ID (NCBI) | 8350 |
RRID | AB_3085214 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P68431 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.
实验方案
Product Specific Protocols | |
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WB protocol for Histone H3 (C-terminal) antibody 68503-1-Ig | Download protocol |
IHC protocol for Histone H3 (C-terminal) antibody 68503-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |