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Histone H3 (C-terminal) Monoclonal antibody, PBS Only

Histone H3 (C-terminal) Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, chicken, zebrafish, wheat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G10C3

Cat no : 68503-1-PBS

Print datasheet

Synonyms

HIST1H3A, 2G10C3, Histone-H3, HistoneH3, Histone H3

验证数据展示

  • WB analysis using 68503-1-Ig (same clone as 68503-1-PBS)

    WB analysis using 68503-1-Ig (same clone as 68503-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68503-1-Ig (HIST1H3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68503-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 68503-1-Ig (same clone as 68503-1-PBS)

    IHC staining of mouse testis using 68503-1-Ig (same clone as 68503-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68503-1-Ig (HIST1H3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68503-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
  • IHC staining of mouse testis using 68503-1-Ig (same clone as 68503-1-PBS)

    IHC staining of mouse testis using 68503-1-Ig (same clone as 68503-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68503-1-Ig (HIST1H3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68503-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

产品信息

68503-1-PBS targets Histone H3 (C-terminal) in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, chicken, zebrafish, wheat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, chicken, zebrafish, wheat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name histone cluster 1, H3a
Synonyms HIST1H3A, 2G10C3, Histone-H3, HistoneH3, Histone H3
Observed Molecular Weight15 kDa
GenBank Accession NumberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP68431
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.