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  • Featured Product
  • KD/KO Validated

Histone H4 Polyclonal antibody

Histone H4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 16047-1-AP

Print datasheet

Synonyms

HIST1H4E, HIST1H4A, H4C5, H4C4, H4C3

验证数据展示

  • WB analysis using 16047-1-AP

    WB analysis using 16047-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • ChIP experiment of HEK-293 using 16047-1-AP

    ChIP experiment of HEK-293 using 16047-1-AP

    Chromatin was prepared from HEK-293 cells. The ChIP was performed with 10 µg of cross-linked chromatin, 5 µg of 16047-1-AP or Normal Rabbit IgG, and 60µl of Protein A sepharose beads. The immunoprecipitated DNA was quantified by real time PCR. Primers are located in the first kb of the transcribed region.

  • IHC staining of mouse small intestine using 16047-1-AP

    IHC staining of mouse small intestine using 16047-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HeLa using 16047-1-AP

    IP experiment of HeLa using 16047-1-AP

    IP result of anti-Histone H4 (IP:16047-1-AP, 3ug; Detection:16047-1-AP 1:600) with HeLa cells lysate 2000ug.

  • IF Staining of HeLa using 16047-1-AP

    IF Staining of HeLa using 16047-1-AP

    Immunofluorescent analysis of HeLa cells using 16047-1-AP (Histone H4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of HEK-293 using 16047-1-AP

    WB analysis of HEK-293 using 16047-1-AP

    WB result of Histone H4 antibody (16047-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Histone H4 transfected HEK-293 cells.

  • IF Staining of HeLa using 16047-1-AP

    IF Staining of HeLa using 16047-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Histone H4 antibody (16047-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HeLa using 16047-1-AP

    IF Staining of HeLa using 16047-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Histone H4 antibody (16047-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of HeLa using 16047-1-AP

    FC experiment of HeLa using 16047-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Histone H4 (16047-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, HT-1080 cells, MCF-7 cells, mouse kidney tissue, mouse thymus tissue, rat thymus tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse small intestine tissue, mouse colon tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive ChIP detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
CHImmunoprecipitation (IP)CHIP :
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16047-1-AP targets Histone H4 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, ChIP, RIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, bovine, yeast
Immunogen Histone H4 fusion protein Ag8999 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name histone cluster 1, H4e
Synonyms HIST1H4E, HIST1H4A, H4C5, H4C4, H4C3
Calculated Molecular Weight 102 aa, 11 kDa
Observed Molecular Weight 14 kDa, 11 kDa
GenBank Accession NumberBC012587
Gene Symbol Histone H4
Gene ID (NCBI) 8367
RRIDAB_2118625
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62805
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

实验方案

Product Specific Protocols
WB protocol for Histone H4 antibody 16047-1-APDownload protocol
IHC protocol for Histone H4 antibody 16047-1-APDownload protocol
IF protocol for Histone H4 antibody 16047-1-APDownload protocol
IP protocol for Histone H4 antibody 16047-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell

Insights into Nucleosome Organization in Mouse Embryonic Stem Cells through Chemical Mapping.

Authors - Lilien N Voong
  • KD Validated
View Article
humanWB

Cell Res

Mutual dependency between lncRNA LETN and protein NPM1 in controlling the nucleolar structure and functions sustaining cell proliferation.

Authors - Xianteng Wang
View Article
mouseWB

Cell Res

SUMO suppresses and MYC amplifies transcription globally by regulating CDK9 sumoylation.

Authors - Fang Yu
View Article
ratWB

Nat Commun

Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.

Authors - Megha Jhanji
View Article
humanWB

Autophagy

The Paf1 complex transcriptionally regulates the mitochondrial-anchored protein Atg32 leading to activation of mitophagy.

Authors - Liangde Zheng
View Article
human,mouseIHC

EMBO J

Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling.

Authors - Harrison Tudor Evans
View Article