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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Histone H4 Polyclonal antibody

Histone H4 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16047

Print datasheet

Synonyms

HIST1H4E, HIST1H4A, H4C5, H4C4, H4C3

验证数据展示

  • IF Staining of HeLa using CL488-16047

    IF Staining of HeLa using CL488-16047

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Histone H4 antibody (CL488-16047) at dilution of 1:200.

  • FC experiment of HeLa using CL488-16047

    FC experiment of HeLa using CL488-16047

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Histone H4 (CL488-16047) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 16047-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16047 targets Histone H4 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Histone H4 fusion protein Ag8999 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name histone cluster 1, H4e
Synonyms HIST1H4E, HIST1H4A, H4C5, H4C4, H4C3
Calculated Molecular Weight 102 aa, 11 kDa
Observed Molecular Weight 14 kDa, 11 kDa
GenBank Accession NumberBC012587
Gene Symbol Histone H4
Gene ID (NCBI) 8367
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62805
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Histone H4 antibody CL488-16047Download protocol
FC protocol for CL Plus 488 Histone H4 antibody CL488-16047Download protocol
Standard Protocols
Click here to view our Standard Protocols