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验证数据展示

  • IF Staining of HeLa using CL488-16047

    IF Staining of HeLa using CL488-16047

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Histone H4 antibody (CL488-16047) at dilution of 1:200.

  • FC experiment of HeLa using CL488-16047

    FC experiment of HeLa using CL488-16047

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Histone H4 (CL488-16047) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Histone H4 Polyclonal antibody

Histone H4 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-16047

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

HIST1H4E, HIST1H4A, H4C5, H4C4, H4C3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 16047-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16047 targets Histone H4 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Histone H4 fusion protein Ag8999 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histone cluster 1, H4e
别名 HIST1H4E, HIST1H4A, H4C5, H4C4, H4C3
计算分子量 102 aa, 11 kDa
观测分子量 14 kDa, 11 kDa
GenBank蛋白编号BC012587
基因名称 Histone H4
Gene ID (NCBI) 8367
RRIDAB_3083984
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62805
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Histone H4 antibody CL488-16047Download protocol
FC protocol for CL Plus 488 Histone H4 antibody CL488-16047Download protocol
Standard Protocols
Click here to view our Standard Protocols