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HLA class I ABC Polyclonal antibody

HLA class I ABC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA and More (4)

Conjugate

Unconjugated

Cat no : 15240-1-AP

Print datasheet

Synonyms

HLA-A, Human leukocyte antigen A, HLA class I histocompatibility antigen, A alpha chain, HLA class I (HLA-A), HLA class I

验证数据展示

  • WB analysis using 15240-1-AP

    WB analysis using 15240-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15240-1-AP (HLA class I ABC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 15240-1-AP

    IHC staining of human tonsillitis using 15240-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15240-1-AP (HLA class I ABC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HLA-A antibody included
  • IHC staining of human tonsillitis using 15240-1-AP

    IHC staining of human tonsillitis using 15240-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15240-1-AP (HLA class I ABC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HLA-A antibody included
  • IF Staining of HepG2 using 15240-1-AP

    IF Staining of HepG2 using 15240-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using HLA class I ABC antibody (15240-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of HepG2 using 15240-1-AP

    IP experiment of HepG2 using 15240-1-AP

    IP result of anti-HLA class I ABC (IP:15240-1-AP, 3ug; Detection:15240-1-AP 1:500) with HepG2 cells lysate 1720ug.

  • IHC staining of human tonsillitis using 15240-1-AP

    IHC staining of human tonsillitis using 15240-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15240-1-AP (HLA class I ABC antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HLA-A antibody included
  • IF Staining of HepG2 using 15240-1-AP

    IF Staining of HepG2 using 15240-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HLA class I ABC antibody (15240-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, HepG2 cells, NCI-H1299 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman tonsillitis tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HLA-A Ready-To-Use IHC Kit. HLA-A primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15240-1-AP targets HLA class I ABC in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA, Cell treatment applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, RIP, Cell treatment
Tested Reactivity human
Cited Reactivityhuman
Immunogen HLA class I ABC fusion protein Ag7370 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name major histocompatibility complex, class I, A
Synonyms HLA-A, Human leukocyte antigen A, HLA class I histocompatibility antigen, A alpha chain, HLA class I (HLA-A), HLA class I
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC003069
Gene Symbol HLA-A
Gene ID (NCBI) 3105
RRIDAB_1557426
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04439
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

实验方案

Product Specific Protocols
WB protocol for HLA class I ABC antibody 15240-1-APDownload protocol
IHC protocol for HLA class I ABC antibody 15240-1-APDownload protocol
IF protocol for HLA class I ABC antibody 15240-1-APDownload protocol
IP protocol for HLA class I ABC antibody 15240-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

IRGQ-mediated autophagy in MHC class I quality control promotes tumor immune evasion

Authors - Lina Herhaus
View Article
humanWB

Nature

Inhibition of PCSK9 potentiates immune checkpoint therapy for cancer.

Authors - Xinjian Liu
View Article
humanWB

Signal Transduct Target Ther

Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis

Authors - Chuan Yang
View Article
humanWB

Cell

Targeting Pin1 renders pancreatic cancer eradicable by synergizing with immunochemotherapy.

Authors - Kazuhiro Koikawa
View Article
human

Nat Cell Biol

An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.

Authors - James W Clancy
View Article
WB

ACS Appl Mater Interfaces

Self-Assembly of a Multifunction DNA Tetrahedron for Effective Delivery of Aptamer PL1 and Pcsk9 siRNA Potentiate Immune Checkpoint Therapy for Colorectal Cancer.

Authors - Wenjing Guo
View Article