CoraLite® Plus 488-conjugated HLA class I ABC Polyclonal antibody
HLA class I ABC Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy HLA-A Ready-To-Use IHC Kit. HLA-A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15240-1-AP
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-15240 targets HLA class I ABC in IF/ICC applications and shows reactivity with human samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | human |
Immunogen | HLA class I ABC fusion protein Ag7370 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | major histocompatibility complex, class I, A |
Synonyms | HLA A, HLAA, HLA-A, MHC class I antigen A*3 |
Calculated Molecular Weight | 41 kDa |
Observed Molecular Weight | 44 kDa |
GenBank Accession Number | BC003069 |
Gene Symbol | HLA-A |
Gene ID (NCBI) | 3105 |
RRID | AB_2919115 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P04439 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 HLA class I ABC antibody CL488-15240 | Download protocol |
FC protocol for CL Plus 488 HLA class I ABC antibody CL488-15240 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |