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验证数据展示

  • WB analysis using 32281-1-AP

    WB analysis using 32281-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32281-1-AP (HLA-DQB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 32281-1-AP

    IHC staining of human tonsillitis using 32281-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 32281-1-AP (HLA-DQB1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 32281-1-AP

    IHC staining of human tonsillitis using 32281-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 32281-1-AP (HLA-DQB1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HLA-DQB1 Polyclonal antibody

HLA-DQB1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32281-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inDaudi cells, Ramos cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32281-1-AP targets HLA-DQB1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 HLA-DQB1 fusion protein Ag34755 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 major histocompatibility complex, class II, DQ beta 1
别名
计算分子量 30 kDa
观测分子量30 kDa
GenBank蛋白编号BC012106
基因名称 HLA-DQB1
Gene ID (NCBI) 3119
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP01920
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The HLA-DQB1 gene is part of the human leukocyte antigen (HLA) complex, which is involved in the immune system's ability to distinguish the body's own proteins from those made by foreign invaders such as viruses and bacteria. It belongs to the MHC class II genes, which provide instructions for making proteins present on the surface of certain immune system cells. These proteins attach to protein fragments (peptides) outside the cell and display them to the immune system. If the immune system recognizes the peptides as foreign, it triggers a response to attack the invading pathogens. .

实验方案

Product Specific Protocols
WB protocol for HLA-DQB1 antibody 32281-1-APDownload protocol
IHC protocol for HLA-DQB1 antibody 32281-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols