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FITC Plus Anti-Human HLA-DR (L243) Rabbit Recombinant Antibody

HLA-DR Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

L243

Cat no : FITC-65503

Print datasheet

Synonyms

HLA DRA, HLA DRA1, HLA-DRA

验证数据展示

  • FC experiment of human PBMCs using FITC-65503

    FC experiment of human PBMCs using FITC-65503

    1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human HLA-DR Rabbit Recombinant Antibody (FITC-65503, Clone:L243) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Lymphocytes and monocytes were gated.

  • FC experiment of human PBMCs using FITC-65503

    FC experiment of human PBMCs using FITC-65503

    1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human HLA-DR Rabbit Recombinant Antibody (FITC-65503, Clone:L243) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Lymphocytes and monocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-65503 targets HLA-DR in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Human lymphoblastoid B-cell line RPMI 8866 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name major histocompatibility complex, class II, DR alpha
Synonyms HLA DRA, HLA DRA1, HLA-DRA
Calculated Molecular Weight 254 aa, 29 kDa
GenBank Accession NumberBC032350
Gene Symbol HLA-DRA
Gene ID (NCBI) 3122
ENSEMBL Gene IDENSG00000204287
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

HLA-DR is a member of MHC class II moleculesencoded by the human leukocyte antigen complex on the region 6p21.31 of chromosome 6. HLA-DR is composed of two non-covalently associated transmembrane glycoproteins (the α and β chains), and is primarily expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelial cells (PMID: 33125816). HLA-DR participate in the antigen presentation to CD4+ or CD8+ T cells and is postulated as a general biomarker of sepsis-induced immunosuppression (PMID: 28199206, 32421903). The Anti-Human HLA-DR antibody, clone L243, is derived from Human lymphoblastoid B-cell line RPMI 8866.

实验方案

Product Specific Protocols
FC protocol for FITC Plus HLA-DR antibody FITC-65503Download protocol
Standard Protocols
Click here to view our Standard Protocols