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HLA-DRB4 Recombinant antibody, PBS Only

HLA-DRB4 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230352F10

Cat no : 83103-1-PBS

Print datasheet

Synonyms

MHC class II antigen DRB4, HLA DRB4, HLA DR4B, 230352F10

验证数据展示

  • WB analysis using 83103-1-RR (same clone as 83103-1-PBS)

    WB analysis using 83103-1-RR (same clone as 83103-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83103-1-RR (HLA-DRB4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83103-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 83103-1-RR (same clone as 83103-1-PBS)

    IHC staining of human tonsillitis using 83103-1-RR (same clone as 83103-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83103-1-RR (HLA-DRB4 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83103-1-PBS in a different storage buffer formulation.

  • IF Staining of Raji using 83103-1-RR (same clone as 83103-1-PBS)

    IF Staining of Raji using 83103-1-RR (same clone as 83103-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DRB4 antibody (83103-1-RR, Clone: 230352F10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83103-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83103-1-RR

    Affinity and Kinetic Characterization of 83103-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83103-1-RR against Human HLA-DRB4 were performed. The affinity constant is 0.623 nM.

产品信息

83103-1-PBS targets HLA-DRB4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, pig
Immunogen HLA-DRB4 fusion protein Ag8492 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name major histocompatibility complex, class II, DR beta 4
Synonyms MHC class II antigen DRB4, HLA DRB4, HLA DR4B, 230352F10
Calculated Molecular Weight 266 aa, 30 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC005312
Gene Symbol HLA-DRB4
Gene ID (NCBI) 3126
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13762
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C