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HLA-DRB4 Recombinant antibody

HLA-DRB4 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230352F10

Cat No : 83103-1-RR

Print datasheet

Synonyms

HLA DR4B, HLA DRB4, HLA-DRB4, MHC class II antigen DRB4

验证数据展示

  • WB analysis using 83103-1-RR

    WB analysis using 83103-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83103-1-RR (HLA-DRB4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Affinity experiments of 83103-1-RR

    Affinity experiments of 83103-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83103-1-RR against Human HLA-DRB4 were performed. The affinity constant is 0.623 nM.

经过测试的应用

Positive WB detected inRaji cells, Ramos cells, Daudi cells, pig spleen tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83103-1-RR targets HLA-DRB4 in WB, ELISA applications and shows reactivity with Human, Pig samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Pig
Immunogen HLA-DRB4 fusion protein Ag8492 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name major histocompatibility complex, class II, DR beta 4
Synonyms HLA DR4B, HLA DRB4, HLA-DRB4, MHC class II antigen DRB4
Calculated Molecular Weight 266 aa, 30 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC005312
Gene Symbol HLA-DRB4
Gene ID (NCBI) 3126
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13762
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HLA-DRB4 antibody 83103-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols