• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

HLA-DRB4 Recombinant antibody

HLA-DRB4 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230352F10

Cat no : 83103-1-RR

Print datasheet

Synonyms

MHC class II antigen DRB4, HLA DRB4, HLA DR4B, 230352F10

验证数据展示

  • WB analysis using 83103-1-RR

    WB analysis using 83103-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83103-1-RR (HLA-DRB4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 83103-1-RR

    IHC staining of human tonsillitis using 83103-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83103-1-RR (HLA-DRB4 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Raji using 83103-1-RR

    IF Staining of Raji using 83103-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DRB4 antibody (83103-1-RR, Clone: 230352F10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83103-1-RR

    Affinity and Kinetic Characterization of 83103-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83103-1-RR against Human HLA-DRB4 were performed. The affinity constant is 0.623 nM.

经过测试的应用

Positive WB detected inRaji cells, Ramos cells, Daudi cells, pig spleen tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRaji cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83103-1-RR targets HLA-DRB4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, pig
Immunogen HLA-DRB4 fusion protein Ag8492 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name major histocompatibility complex, class II, DR beta 4
Synonyms MHC class II antigen DRB4, HLA DRB4, HLA DR4B, 230352F10
Calculated Molecular Weight 266 aa, 30 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC005312
Gene Symbol HLA-DRB4
Gene ID (NCBI) 3126
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13762
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HLA-DRB4 antibody 83103-1-RRDownload protocol
IHC protocol for HLA-DRB4 antibody 83103-1-RRDownload protocol
IF protocol for HLA-DRB4 antibody 83103-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols