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验证数据展示

  • IF Staining of human tonsillitis using CL594-66530

    IF Staining of human tonsillitis using CL594-66530

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using the CoraLite®594-conjugated version of this antibody, CL594-66530 (HLA-E antibody), at dilution of 1:100.

  • IF Staining of human tonsillitis using CL594-66530

    IF Staining of human tonsillitis using CL594-66530

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using the CoraLite®594-conjugated version of this antibody, CL594-66530 (HLA-E antibody), at dilution of 1:100.

CoraLite®594-conjugated HLA-E Monoclonal antibody

HLA-E Monoclonal Antibody for IF-P
Cat No. CL594-66530

产品说明书

CloneNo. 1A4G3

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

IF-P

别名

DKFZp686P19218, EA1.2, EA2.1, HLA 6.2, HLA E, HLAE, HLA-E, MHC, MHC class I antigen E, QA1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy HLA-E Ready-To-Use IHC Kit. HLA-E primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66530-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66530 targets HLA-E in IF-P applications and shows reactivity with Human samples.

经测试应用 IF-P Application Description
经测试反应性 Human
免疫原 HLA-E fusion protein Ag6724 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 major histocompatibility complex, class I, E
别名 DKFZp686P19218, EA1.2, EA2.1, HLA 6.2, HLA E, HLAE, HLA-E, MHC, MHC class I antigen E, QA1
计算分子量 40 kDa
GenBank蛋白编号BC002578
基因名称 HLA-E
Gene ID (NCBI) 3133
RRIDAB_2883599
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP13747
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-E is a non-classical MHC class I molecule. HLA-E is frequently overexpressed in tumor diseases, transplants and virus-infected cells and represents an immunomodulatory molecule by binding to the receptors CD94/NKG2A, -B and -C on NK and T cells. Due to its immune suppressive features HLA-E expression might represent an important mechanism of tumors to escape immune surveillance.(PMID: 667938; 3375250; 2249951; 27589686)

实验方案

Product Specific Protocols
IF protocol for CL594 HLA-E antibody CL594-66530Download protocol
Standard Protocols
Click here to view our Standard Protocols