HLA-F Monoclonal antibody
HLA-F Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1B12C2
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, NCCIT cells, Raji cells, human peripheral blood leukocytes |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | Raji cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:8000-1:32000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66819-1-Ig targets HLA-F in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Tested Reactivity | Human |
Immunogen | HLA-F fusion protein Ag6475 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | major histocompatibility complex, class I, F |
Synonyms | HLA F antigen, HLA F, HLA CDA12, HLA 5.4, CDA12 |
Calculated Molecular Weight | 39 kDa |
Observed Molecular Weight | 40-45 kDa |
GenBank Accession Number | BC062991 |
Gene Symbol | HLA-F |
Gene ID (NCBI) | 3134 |
RRID | AB_2882162 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P30511 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for HLA-F antibody 66819-1-Ig | Download protocol |
IHC protocol for HLA-F antibody 66819-1-Ig | Download protocol |
IF protocol for HLA-F antibody 66819-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |