验证数据展示
经过测试的应用
| Positive IF/ICC detected in | Raji cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66819 targets HLA-F in IF/ICC applications and shows reactivity with Human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human |
| 免疫原 | HLA-F fusion protein Ag6475 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | major histocompatibility complex, class I, F |
| 别名 | HLA F antigen, HLA F, HLA CDA12, HLA 5.4, CDA12 |
| 计算分子量 | 39 kDa |
| 观测分子量 | 40-45 kDa |
| GenBank蛋白编号 | BC062991 |
| 基因名称 | HLA-F |
| Gene ID (NCBI) | 3134 |
| RRID | AB_3672923 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P30511 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 HLA-F antibody CL488-66819 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |