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HLA-G Monoclonal antibody, PBS Only

HLA-G Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E5A10

Cat no : 66447-1-PBS

Print datasheet

Synonyms

HLA 6.0, HLA G, HLA G antigen, HLAG, HLA-G, MHC class I antigen G, MHC G

验证数据展示

  • WB analysis of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

    WB analysis of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66447-1-Ig (HLA-G Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66447-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

    IHC staining of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66447-1-Ig (HLA-G Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66447-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HLA-G antibody included
  • IHC staining of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

    IHC staining of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66447-1-Ig (HLA-G Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66447-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HLA-G antibody included
Planning an IHC experiment? We recommend our IHCeasy HLA-G Ready-To-Use IHC Kit. HLA-G primary antibody included.

产品信息

66447-1-PBS targets HLA-G in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen HLA-G fusion protein Ag10839 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name major histocompatibility complex, class I, G
Synonyms HLA 6.0, HLA G, HLA G antigen, HLAG, HLA-G, MHC class I antigen G, MHC G
Calculated Molecular Weight 338 aa, 38 kDa
Observed Molecular Weight 33-45 kDa
GenBank Accession NumberBC021708
Gene Symbol HLA-G
Gene ID (NCBI) 3135
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP17693
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). HLA-G is a non-classical MHC class I molecule with multiple immunoregulatory properties. HLA-G exhibits a restricted pattern of expression that includes placental extravillous trophoblasts at the maternal-fetal interface, where it abolishes maternal immune cell activity against fetus and establishes immune tolerance. Aberrant expression of HLA-G has been found in a variety of human neoplastic diseases. It plays an important role in the escape of tumor cells from immunosurveillance.